Construction and Antibacterial Effect Evaluation of Nano TiO2 Loaded Moxifloxacin Hydrochloride System
HUANG Han-Ye, GUO Xue-Ping, XU Jia-Wei, LI Qian-Qian, CAI Xin-Kai, GUAN Xiong, PAN Xiao-Hong*
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Lab of Ministry of Education for Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract：Moxifloxacin hydrochloride (MOX) is a broad-spectrum quinolone drug and is widely used in clinic as a antibacterial agent. In order to explore the effect of nanocarriers on its antibacterial effect, MOX was loaded onto nano titanium dioxide (TiO2) by surface adsorption in this study, and then the chemical characterization, antibacterial activity against Escherichia coli and pathological observation of the nano TiO2-MOX were investigated. Nano TiO2 combined with MOX in the form of physical embedding. When the content of nano TiO2 increased from 0.5 mg to 1.0 mg, the drug loading and the encapsulation rate increased by about 20% and 40% respectively. Nano TiO2 in nano TiO2-MOX could help release the effective components of MOX. The results of the in vitro activity against E. coli showed that the synthesized nano TiO2 had certain antibacterial activity (22.8%), and the antibacterial activity of MOX increased from 28.0% to 73.4% with the support of nano TiO2. Pathological observation also indicated that the damage of the cell membrane of E. coli was not obvious by MOX, but nano TiO2 and nano TiO2-MOX could cause obvious damage to the cell membrane and then cause cell death. In this study, the synthesized nano TiO2-MOX showed good drug loading and releasing effect, and significantly improved the antibacterial activity of MOX, which provides a scientific basis for the application of nano TiO2 as a new drug carrier in further clinical research.
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