Pharmacodynamics Study of Compound Huang-Long Cream in Promoting the Repair of Acute Soft Tissue Injury and Scald Injury
XIE Tong1, ZHANG Pei-Hua1, LIN Jia-Hao1,2,*
1 College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; 2 Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, Beijing 100193, China
Abstract：Acute soft tissue injury and scald are common types of traumatic diseases in veterinary clinics, both characterized by local acute aseptic inflammatory response and tissue damage repair as the main pathological changes. In the field of veterinary medicine, there is not much research on drugs that promote tissue damage repair at present. Compound Huang-Long Cream is a Chinese herbal ointment for promoting tissue damage repair according to the empirical formula from Traditional Chinese Veterinary Medicine, which has a good clinical efficacy. This study aimed to conduct a preliminary exploration of the therapeutic effect of Compound Huang-Long Cream and provide a experimental basis for its development and clinical application. An acute soft tissue injury model in SD rats (Rattus norvegicus) and a scald model in C57BL/6J mice (Mus musculus) with high-temperature mental scald were established. The changes of symptomatic manifestations and the haematoxylin and eosin (HE) staining were used to evaluate the pharmacodynamics efficacy of Compound Huang-Long Cream in promoting the repair of acute soft tissue injury and scald injury. The results showed that in the acute soft tissue injury repair experiment, the syndrome score of rats with acute soft tissue injury decreased extremely significantly (P<0.01) and the swelling rate of hind limbs decreased extremely significantly (P<0.01) in the Compound Huang-Long Cream group compared with the model group at 72 h after molding. There was a significant difference (P<0.05) in the swelling rates in the Compound Huang-Long Cream group compared with the positive drug （Diclofenac Diethylamine Emulgel） group. In the scald injury repair experiment, the healing rate of scald focus in the Compound Huang-Long Cream group was (47.82±5.53)%, which was 1.4 times higher than that in the model group (P<0.05) on the day 7. The pathological section results showed that compared with the model group, hemorrhage and inflammation were reduced and collagen fibers were increased in both the soft tissue of rats and the skin of mice in the Compound Huang-Long Cream group. This study indicates that Compound Huang-Long Cream can significantly improve the symptoms of ecchymosis and edema, inhibit inflammation and promote the repair of damaged tissues in rats with acute soft tissue injury model. Compared with diclofenac diethylamine emulgel, it was better at eliminating edema. In terms of promoting scald repair, Compound Huang-Long Cream could accelerate the healing of scald wounds in scald model mice, promoted their wound repair, and inhibited inflammation. This study provides a preliminary experimental basis for the clinical application of Compound Huang-Long Cream.
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