Abstract：The shift of soil microbial community is one of the main factors affecting continuous cropping. It has been observed that continuous cropping obstacles exist in celery (Apium graveolens) cultivation. However, the effects of continuous cropping of celery on rhizosphere soil microbial community remain unclear. In this study, Illumina MiSeq was used to study the shift of bacterial and fungal communities in the rhizosphere of celery after continuous cropping. The results showed that after continuous monoculture, the rhizosphere soil bacteria richness increased but the diversity did not change significantly, while the abundance and diversity of fungi decreased significantly. Taxonomic analysis further showed that Proteobacteria, Actinobacteriota, Acidobacteriota and Chloroflexi were the dominant phyla in all the sample bacterial communities, while Ascomycota was the absolute dominant phyla. After continuous cropping, the rhizosphere soil fungal community richness of diseased plants decreased significantly compared with that of healthy plants (P<0.05), decreased significantly compared with the soil without celery planting (P<0.01). In addition, LEfSe (linear discriminant analysis effect size) analysis results showed that the beneficial bacteria genera were significantly enriched in the healthy, diseased and uncultivated soils samples of the same field. Meanwhile, the significant increase (P<0.05) of the abundance of pathogenic fungus Stemphylium sp. in the rhizosphere of diseased plants indicated that the fungus might be an important factor causing continuous cropping disorder of celery. This study revealed the shift of bacterial and fungal community diversity and composition in celery rhizosphere soil under continuous cropping, which would be helpful to understand the microecological environment of celery rhizosphere soil under continuous cropping.
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