Heterogeneity Analysis of Milk Exosomes of the Mammary Gland in Different Functional States of the Dairy Cows (Bos taurus)
YANG Fang-Ling1, WANG Yu1, WU Shuai-Shuai2, WANG Jian-Fa3, ZHU Hai-Jing1, LI Long-Ping1, QU Lei1, DONG Shu-Wei1,*
1 College of Life Sciences, Yulin University, Yulin 719000, China; 2 College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; 3 College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract：Mastitis is the most important disease affecting dairy cows (Bos taurus), but there is no efficient method for its prevention and control, and exosomes in cow milk may have an important role in the development of mastitis. To investigate how exosomes in milk of cows change in different functional states, such as healthy, subclinical mastitis and clinical mastitis, in this study, exosomes were isolated from the milk of cows by ultracentrifugation, identified by nanoparticle tracking, transmission electron microscopy and Western blot, and compared the differences in particle diameter, number, protein concentration and surface marker molecules between the groups. The results showed that the particle size of exosomes in each group was close, the morphology was consistent with the characteristics of exosomes, and there was no significant difference between the groups; Western blot results showed that the exosome molecular marker proteins cluster of differentiation 9 (CD9), CD81 and tumor susceptibility gene 101 (TSG101) were positive in each group. The most exosomal particles were found in the subclinical mastitis group, followed by the clinical mastitis group, both of which were significantly higher than the healthy group (P<0.05); the highest exosomal protein content was found in the subclinical mastitis group, followed by the clinical mastitis group, both of which were significantly higher than the healthy group (P<0.05). In this study, exosomes were isolated from cow milk, and their number, protein content and surface molecular markers changed significantly in different functional states of the mammary gland, suggesting that exosomes may have a regulatory role in the immune process against infection in the mammary gland of cows. Exosomes may play a role in the regulation of mammary gland immunity against infection in dairy cows.
杨芳玲, 王玉, 吴帅帅, 王建发, 朱海鲸, 李陇平, 屈雷, 董书伟. 奶牛乳腺在不同机能状态下乳汁外泌体的异质性分析[J]. 农业生物技术学报, 2023, 31(10): 2098-2105.
YANG Fang-Ling, WANG Yu, WU Shuai-Shuai, WANG Jian-Fa, ZHU Hai-Jing, LI Long-Ping, QU Lei, DONG Shu-Wei. Heterogeneity Analysis of Milk Exosomes of the Mammary Gland in Different Functional States of the Dairy Cows (Bos taurus). 农业生物技术学报, 2023, 31(10): 2098-2105.
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