Expression of AmFIM Gene in Solanum nigrum and Its Effect on Flower Development
FENG Li-Jun1, CHEN Ke-Li1, HAO Yan-Min1, LI Fei-Fei1, CUI Min-Long1,2, PIAO Chun-Lan1,2*
1 College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China; 2 Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province/College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
Abstract：The unusual floral organs (UFO) gene and its orthologous genes have an important role in the regulating the identity of floral meristem and the development of floral organs, and its functions have been revealed in many species. AmFimbriata (AmFIM) is the earliest cloned UFO like gene, compared with other UFO like genes, it has certain differences in function and gene interaction. In addition, the study of AmFIM in Antirrhinum majus was limited to transposon-mediated mutants. Therefore, the function of AmFIM gene needs further verification and improvement. In this experiment, the pBI35S:: AmFIM overexpression vector was heterologously transformed in Solanum nigrum, the morphological observation found that the transgenic S. nigrum plants showed obvious dwarf, early flowering phenotype, and the difference in AmFIM gene expression had different effects on S. nigrum cymes and floral organs. In weakly phenotypic lines, the number of flowers in each inflorescence was reduced, the apical inflorescence architecture was complicated, and the floral organs were not significantly different compared with the wild type; in strongly phenotypic lines, the cymes were transformed into single flowers, the floral organs were homozygously transformed, the sepals became larger, petaloid tissue appeared in sepals and stamens, staminoid tissue appeared in carpels, and sepals fused with petals, petals fused with stamens. The internal structure of stamenloid carpel of transgenic S. nigrum was further observed by paraffin section, and it was found that there were tissues similar to anthers and pollen. The results showed that the overexpression of AmFIM gene in S. nigrum affects the inflorescence architecture and the development of floral organs, reflecting the function of AmFIM gene in regulating the determinacy of floral meristems, floral organ differentiation and maintaining floral organ boundaries. This study further improves the research content of AmFIM gene and provides a reference for the molecular regulation mechanism of UFO like genes.
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