Correlation Analysis Between Amino Acid Content in Bovine (Bos taurus) Seminal Plasma and Sperm Motility
LI Xin, HAN Bi-Ying, YANG Ming, ZHANG Xue-Li, HAI Chao, LI Guang-Peng, ZHAO Yue-Fang*
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010070, China
Abstract：Cryopreservation of semen plays a vital role in accelerating genetic improvement and elite breeding, but it has a detrimental effect on sperm quality. In order to explore the key factors related to sperm freezing tolerance in seminal plasma, in this study, the semen of 5 Luxi cattle (Bos taurus) and 5 Mongolian cattle were collected, semen volume, sperm density, sperm motility and other indicators were systematically detected and analyzed, and the contents of various amino acids in seminal plasma were quantitatively analyzed by HPLC. The results showed that the average semen volume of 10 cattle was 4.85 mL, the average sperm density was 1.376 billion pieces/mL, the vitality of fresh sperm and frozen sperm were 69.67% and 35.68% respectively, and the concentration of total amino acids in seminal plasma ranged from 25 to 54 mg/mL, and Mongolian cattle were higher than Luxi cattle; The correlation analysis between the concentration of amino acids in seminal plasma and sperm motility showed that the concentrations of Tyr, Phe, Ser, Arg, Thr and Cys were significantly correlated with frozen sperm motility, and Pearson correlation coefficient r values were 0.743, 0.755, 0.662, 0.649, 0.725 and 0.729, respectively (P<0.05), and the concentration of Pro was extremely significantly correlated with frozen sperm motility (r=0.768, P<0.01). In conclusion, the detection of amino acid content in seminal plasma can be used as a potential index to evaluate the freezing tolerance of bovine sperm. This study provides theoretical basis for further improving the quality of frozen sperm.
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