Abstract：In the practice of genetic engineering, such as improving the nutritional quality of seeds and using seed-based bioreactor to produce proteins with industrial or medicinal value, it is necessary to use seed specific promoters to drive the transcription of exogenous genes. Seed storage proteins are the general name of a large number of storage proteins synthesized and stored in the process of seed ripening in higher plants, the promoters of the genes coding seed storage proteins serve as an important source for seed specific promoters. In order to obtain the seed specific promoter of Medicago truncatula, 2 075 bp promoter sequence (named PMt1g072600) of upstream of coding region of the major storage protein legumin gene Mt1g072600 was cloned, and the putative cis-acting elements of the promoter were predicted using workflow embed in PLACE and PlantCARE, the results showed that this sequence contained E-box, A/T rich element, P-box and other cis-acting elements with highly relevant features to seed specific promoter. The plant expression vector to drive the GUS gene using this promoter was constructed and transformed into Arabidopsis thaliana, the result of analysis of GUS expression level in transgenic A. thaliana seeds showed that the expression of GUS driven by PMt1g072600 was significantly higher than that drived by PCaMV35S. The results of histochemical staining of tissues/organs of transgenic A. thaliana showed that GUS driven by PMt1g072600 showed the specificity of seed expression.This study identified a seed specific promoter PMt1g072600 of M. truncatula, which can be used to drive the specific transcription of exogenous genes in transgenic plant seeds. This study also provides a reference for selection and utilization of seed specific promoters in plant genetic engineering.
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