Abstract：Abstract The growth and development of stem could be induced and changed by the infection of Ustilago esculenta in Zizania latifolia, but the regulatory of swollen stem is still unclear. In this research, Z. latifolia WRKY transcription factor 72 (ZlWRKY72) (GenBank number: MG365889) was cloned with full DNA length of 2 831 bp with one intron. The full length of its cDNA was 1 085 bp with a 738 bp of ORF coding frame encoding 245 amino acids, the typical structure domain of WRKYGQK and a zinc finger structure of Cys2.His2 were found in ZlWRKY72. The phylogenetic analysis showed that the ZlWRKY72 has the highest homology with Oryza sativa subsp. japonica OsWRKY72. Expression analysis showed that the higher expressions of ZlWRKY72 during the development of swollen stem of Z. latifolia, liking the higher expression in leaves than stems at tillering stage seedling (P＜0.05), while the higher expression in stems than leaves at stage of initiation swollen using qRT-PCR (P＜0.05). The expression of ZlWRKY72 in stem was related to the successfully infection and reproduction of U. esculenta. After the initiation of swollen stem, the higher expression of ZlWRKY72 were detected in the stem of Jiaobai than grey Jiaobai (P＜0.05), which was reduced significantly during the development of swollen stem in Jiaobai (P＜0.05). The expression of ZlWRKY72 were higher in male Z. latifolia than Jiaobai during the initiation of swollen. It was found that the reduction expression of ZlWRKY72 occurred following with the increasing hypha reproduction of U. esculenta in stem. The expression responses and regulation of ZlWRKY72 are investigated and discussed during the development of swollen stem in Z. latifolia, which might be used in the research of swollen stem. It provides a theoretical basis for studying the mechanism of swelling development of Zizania latifolia.
[1]陈守良, 徐克学.菰属Zizania L.植物的分支分类研究[J].植物研究, 1994, 14(4):385-394[2]丁小余, 陈维培, 徐祥生.茭白分蘖苗端的结构及发育研究[J].南京师大学报, 1991, 14(2):60-68[3]韩秀芹.2004.茭白黑粉菌生物学特性研究[D].硕士学位论文, 扬州大学, 导师:曹碚生, 江解增, pp.11-12.(Han X Q.2004. Studies on Biological Characteristics of Ustilago Esculenta[D]. Thesis for M.S., Yangzhou University, Suppervisor:Cao B S, Jiang J Z, pp.11-12.)[4]江解增, 邱届娟, 韩秀芹, 等.茭白生育过程中地上各部位内源激素的含量变化[J].植物科学学报, 2004, 22(30):245-250[5]金晔.2015.茭白茎部膨大前后应激响应的蛋白质组分析[D].硕士学位论文, 中国计量大学, 导师:叶子弘, pp.49-50.(Jin Y. 2015. Proteomic analysis of the stress response in pre- and post- swollen stem in Zizania latifolia[D]. Thesis for M.S., China Jingliang University, Suppervisor:Ye Z H, pp.49-50.)[6]康璐瑶, 邢阿宝, 崔海峰, 等.ZlADR1基因的克隆及其在茭白孕茭中的表达[J].农业生物技术学报, 2017, 25(11):1799-1808[7]柯卫东, 孔庆东, 余家林.双季茭白品种资源材料的系统聚类分析[J].华中农业大学学报, 1997, (46):598-602[8]李蕾, 谢丙炎, 戴小枫, 等.WRKY转录因子及其在植物防御反应中的作用[J].分子植物育种, 2005, 3(3):401-408[9]李帅.2016.茭白Zl-RPM1和Zl-ADR1抗病基因的克隆及表达分析[D].硕士学位论文, 中国计量大学, 导师:叶子弘, pp.1-2.(Li S. 2016. Cloning and expression analysis of Zl-RPM1 and Zl-ADR1 resistance genes in Zizania latifolia[D]. Thesis for M.S., China Jingliang University, Suppervisor: Ye Z H, pp.1-2.)[10]宋钰, 刘冬梅, 余迪求.高表达水稻WRKY72基因影响拟南芥生长素转导[J].云南植物研究, 2008, 30(6):699-705[11]宋钰, 荆邵娟, 余迪求.水稻WRKY转录调控因子基因功能研究进展[J].中国水稻科学, 2009, 23(5):477-455[12]谢政文, 王连军, 陈锦洋, 等.植物WRKY转录因子及其生物学功能研究进展[J].中国农业科技导报, 2016, 18(3):46-54[13]邢阿宝, 崔海峰, 俞晓平, 等.茭白茎部CTK及ABA含量动态变化分析[J].中国计量大学学报, 2017, 28(2):261-268[14]闫宁, 薛惠民, 石林豫, 等.茭白“雄茭”和“灰茭”的形成及遗传特性[J].中国蔬菜, 2013, (16):35-42[15]应荣, 崔海峰, 倪方群, 等.杀菌剂敌磺钠及植物生长调节剂对茭白孕茭的影响[J].植物生理学报, 2014, 50(7):946-952[16]尤文雨, 叶子弘, 刘倩, 等.我国茭白的生物学研究[J].长江蔬菜, 2008, (11):35-38[17]尤文雨, 邹克琴, 俞晓平, 等.菰黑粉菌的分离鉴定及其发酵液中植物激素的检测[J].菰黑粉菌的分离鉴定及其发酵液中植物激素的检测菌物学报, 2010, (2):178-184[18]Chan Y S, Thrower L B.The host-parasite relationship between Zizania caduciflora Turcz. and Ustilago esculenta P. Henn. IV. growth substances in the host-parasite combination[J].New Phytologist, 1980, 85(2):225-233[19]Cheng Y, Zhou Y, Yang Y, et al. Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors[J].Plant Physiology, 2012, 159 (2):810-825[20]Choi C, Wang S H, Fang I R, et al. Molecular characterization of Oryza sativa WRKY6, which binds to Wbox-like element 1 of the Oryza sativa pathogenesis-related(PR) 10a promoter and confers reduced susceptibility to pathogens[J].New Phytologist, 2015, 208(3):846-859[21]Chi Y, Yang Y, Zhou Y, et al.Protein-protein interactions in the regulation of WRKY transcription factors [J].Molecular Plant, 2013, 6(2):287-300[22]Chung K R, Tzeng D D.Nutritional requirements of the edible gall-producing fungus Ustilago esculenta [J].Journal of Biological Sciences, 2004, 4(2):246-252[23]Eulgem T, Rushton P J, Robatzek S, et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science, 5(5):199-206.[J].Trends in Plant Science, 2000, 5(5):199-206[24]Gl?ckner G, Eichinger L, Szafranski K, et al.Sequence and analysis of chromosome 2 of Dictyostelium discoideum [J].Nature, 2002, 418(6893):79-85[25]Hu L, Wu Y, Wu D, et al.The Coiled-coil and nucleotide binding domains of brown planthopper resistance 14 function in signaling and resistance against planthopper in Rice[J].Plant Cell, 2017, 29(10):-[26]Li R, Zhang J, Li J C, et al.Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores[J].ELife, 2015, 4:-[27]Liu X Q, Bai X Q, Wang X J, et al.OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J].Cell Research, 2005, 15:593-603[28]Liu X Q, Bai X Q, Wang X J, et al.OsWRKY71, a rice transcription factor, is involved in rice defense response[J].Journal of Plant Physiology, 164:969-979.[J].Journal of Plant Physiology, 2007, 164:969-979[29]Luo X, Sun X, Liu B, et al.Ectopic expression of a WRKY homolog from glycine soja alters flowering time in Arabidopsis[J].Plos One, 2013, 8(8):-[30]Mangelsen E, Kilian J, Berendzen K W, et al.Phylogenetic and comparative gene expression analysis of barley ( Hordeumvulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots[J].Bmc Genomics, 2008, 9(1):194-[31]Pandey S P, Somssich I E.The role of WRKY transcription factors in plant immunity[J].Plant Physiology, 2009, 150(4):1648-1655[32]Raineri J, Wang S, Peleg Z, et al.The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress [J].Plant Molecular Biology, 2015, 88(4-5):401-413[33]Schmutz J, Cannon S B, Schlueter J, et al.Genome sequence of the palaeopolyploid soybean [J].Nature, 2010, 463(7278):178-183[34]Sun Y, Yu D.Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement[J].Plant Cell Reports, 2015, 34(8):1295-1306[35]Wang F, Chen H W, Li Q T, et al.GmWRKY27 interacts with GmMYB174 to reduce expression of GmNAC29 for stress tolerance in soybean plants[J].Plant Journal, 2015, 83(2):224-236[36]Xie Z, Zhang Z L, Zou X L, et al.Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells[J].Plant Physial, 2005, 137(1):176-189[37]Xie Z, Zhang Z L, Zou X L, et al.Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells[J].Plant Journal, 6(2):231-242.[J].Plant Journal, 2006, 6(2):231-242[38]Yu Y, Hu R, Wang H, et al.MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering[J].Plant Science, 2013, 212:1-9[39]Zhang Z L, Shin M, Zou X, et al.A negative regulator encoded by a rice WRKY gene repressing both abscisic-acid and gibberellin signaling in aleurone cells[J].Plant Molecular Biology, 2009, 70(1-2):139-151[40]Zhang J Z, Chu F Q, Guo D P, et al.The vacuoles containing multivesicular bodies:a new observation in interaction between Ustilago esculenta and Zizania latifolia[J].European Journal of Plant Pathology, 138:79-91.[J].European Journal of Plant Pathology, 2014, 138:79-91
