Abstract：Agouti gene is one of the important influencing animal coat color genes, although the expression of Agouti gene was found in a variety of animal, Agouti protein (Agouti signaling protein, ASIP) reports was rare on effects of Alpaca (Lama pacos) proliferation of melanocytes and genes related to coat color. In order to observe effect of ASIP-YY (a C-terminal fragment of ASIP) on melanocytes and the formation of Alpaca skin melanin，the different concentrations of ASIP-YY was used in extra-organismal melanocytes of Alpaca skin. Different concentrations of ASIP-YY (0.0, 0.1, 1.0, 10.0, 100.0 nmol/L) treated melanocytes of Alpaca Skin at 72 h. The activity of melanocytes were detected using methyl thiazolyl tetrazolium (MTT) method, the melanin content was tested with ultraviolet spectrophotometric method and qRT-PCR was used to detect the expressions of TYR, TRP-1, TRP-2, MC1R and MTIF mRNA. The results showed that the melanocytes activity was inhibited; the expression of TYR mRNA was significantly inhibited by 0.1,10.0, 100.0 nmol/L ASIP-YY(P＜0.01), the expression of TRP-1 mRNA was significantly inhibited by 10.0, 100.0 nmol/L ASIP-YY(P＜0.01), the expression of MC1R mRNA was inhibited by 10.0 nmol/L ASIP-YY (P＜0.05), the expression of MITF mRNA was significantly inhibited by 0.1, 1.0, 10.0 nmol/L ASIP-YY(P＜0.01), 0.1, 1.0 nmol/L ASIP-YY promoted the expression of TRP-1, TRP-2 mRNA(P＜0.05), 1.0, 100.0 nmol/L ASIP-YY significantly promoted the expression of MC1R mRNA(P＜0.01). ASIP-YY can make the size of melanocytes to be smaller and inhibit the expression of melanin related gene. ASIP-YY may be competitive with MC1R to combine, so as to inhibit the expression of melanin related genes. This study provides a theoretical basis for further study on the regulation of ASIP on Alpaca skin melanin formation.
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