联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月3日 星期日
  2017, Vol. 25 Issue (7): 1072-1077    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
ASIP-YY对羊驼皮肤黑色素细胞形态和黑色素生成的影响
南瑞鹏1,牛峰2,李振2,卢杰丽2,庞全海3
1. 山西农业大学动物科技学院
2. 山西农业大学 动物科技学院
3. 山西农业大学
Influence of ASIP-YY on Morphology and Melanogenesis of Alpaca (Lama pacos) Skin Melanocytes
全文: PDF (3758 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 鼠灰色(Agouti)基因时影响动物毛色的重要基因之一,虽然在多种动物中发现了Agouti基因的存在和表达,但Agouti信号蛋白(Agouti signaling protein, ASIP)对羊驼(Lama pacos)黑色素细胞的增殖及对毛色相关基因的影响研究鲜见报道。本研究旨在利用不同浓度ASIP-YY (a C-terminal fragment of ASIP)作用于体外培养的羊驼皮肤黑色素细胞,观察其对黑色素细胞及黑色素生成的影响。不同浓度ASIP-YY(0.0、0.1、1.0、10.0、100.0 nmol/L)处理羊驼皮肤黑色素细胞72 h。四甲基偶氮噻唑蓝(methyl thiazolyl tetrazolium, MTT)法检测黑色素细胞活力,紫外分光光度法检测黑色素含量,qRT-PCR检测酪氨酸酶(Tyrosinase, TYR)、TRP-1、TRP-2、黑色素细胞膜上的黑色素皮质激素受体1(melanocortin-1 receptor, MC1R)和小眼畸形相关转录因子(Micropht-halmiaassocitated transcription factor, MITF) mRNA表达量。结果显示,黑色素细胞活力受到抑制;ASIP-YY在0.1、10.0、100.0 nmol/L时对TYR mRNA的表达极显著抑制(P<0.01),10.0、100.0 nmol/L时对TRP-1 mRNA的表达极显著抑制(P<0.01),10.0 nmol/L时对MC1R mRNA的表达显著抑制(P<0.05),0.1、1.0、10.0 nmol/L时对MITF mRNA的表达极显著抑制(P<0.01);0.1、1.0 nmol/L时对TRP-1、TRP-2 mRNA的表达显著提高(P<0.05),1.0、100.0 nmol/L时对MC1R的表达极显著提高(P<0.01)。ASIP-YY可使黑色素细胞体积变小,抑制黑色素相关基因的表达,可能是ASIP-YY竞争性的与MC1R结合,从而抑制黑色素相关基因的表达。本实验为进一步研究ASIP对羊驼皮肤黑色素生成调控提供一定的理论基础。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
南瑞鹏
牛峰
李振
卢杰丽
庞全海
关键词 ASIP-YY羊驼黑色素细胞黑色素    
Abstract:Agouti gene is one of the important influencing animal coat color genes, although the expression of Agouti gene was found in a variety of animal, Agouti protein (Agouti signaling protein, ASIP) reports was rare on effects of Alpaca (Lama pacos) proliferation of melanocytes and genes related to coat color. In order to observe effect of ASIP-YY (a C-terminal fragment of ASIP) on melanocytes and the formation of Alpaca skin melanin,the different concentrations of ASIP-YY was used in extra-organismal melanocytes of Alpaca skin. Different concentrations of ASIP-YY (0.0, 0.1, 1.0, 10.0, 100.0 nmol/L) treated melanocytes of Alpaca Skin at 72 h. The activity of melanocytes were detected using methyl thiazolyl tetrazolium (MTT) method, the melanin content was tested with ultraviolet spectrophotometric method and qRT-PCR was used to detect the expressions of TYR, TRP-1, TRP-2, MC1R and MTIF mRNA. The results showed that the melanocytes activity was inhibited; the expression of TYR mRNA was significantly inhibited by 0.1,10.0, 100.0 nmol/L ASIP-YY(P<0.01), the expression of TRP-1 mRNA was significantly inhibited by 10.0, 100.0 nmol/L ASIP-YY(P<0.01), the expression of MC1R mRNA was inhibited by 10.0 nmol/L ASIP-YY (P<0.05), the expression of MITF mRNA was significantly inhibited by 0.1, 1.0, 10.0 nmol/L ASIP-YY(P<0.01), 0.1, 1.0 nmol/L ASIP-YY promoted the expression of TRP-1, TRP-2 mRNA(P<0.05), 1.0, 100.0 nmol/L ASIP-YY significantly promoted the expression of MC1R mRNA(P<0.01). ASIP-YY can make the size of melanocytes to be smaller and inhibit the expression of melanin related gene. ASIP-YY may be competitive with MC1R to combine, so as to inhibit the expression of melanin related genes. This study provides a theoretical basis for further study on the regulation of ASIP on Alpaca skin melanin formation.
Key wordsASIP-YY    Alpaca (Lama pacos)    Melanocytes    Melanin
收稿日期: 2016-12-23      出版日期: 2017-06-16
基金资助:国家自然科学基金项目
通讯作者: 庞全海     E-mail: pangquanhai@126.com
引用本文:   
南瑞鹏 牛峰 李振 卢杰丽 庞全海. ASIP-YY对羊驼皮肤黑色素细胞形态和黑色素生成的影响[J]. , 2017, 25(7): 1072-1077.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I7/1072
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn