Abstract:Research results of miniature pig inbred cultivation and application, which were achieved by the scientists from Institute of Animal Sciences of Chinese Academy of Agricultural Sciences (CAAS). By comprehensive measures of "inbreeding", improving the level of nutrition, estrus synchronization, and cage rearing technology et al., which lasted 15 years, they bred international's first inbred pigs with two Wuzhishan mini-pig(WZSP)(Sus scrofa) as progenitor, gradually overcame three stages problems as high offspring malformation rate, high weakling piglet rate and low survival rate. This article describes the breeding process, inbred line identification, germplasm characteristics of the new genetic resources, and their development and utilization et al. The results fill the gaps for pig inbred line in domestic and foreign research, and provide a new vision for animal inbreeding theory and applied research in large mammals inbred line.