摘要为了解大麦(Hordeum vulgare)亲本材料遗传多样性，筛选与大麦条纹病性状相关联的SSR标记，利用100对多态性SSR引物对86个大麦品种进行分析，共检测出269个等位变异，平均每对引物为2.69个，变幅为2~5；等位基因频率为0.012~0.988；Shannon指数为0.064~1.385；遗传相似系数为0.547~0.955；多态性信息含量(polymorphic information content, PIC)为0.023~0.737，平均为0.357。群体遗传结构分析表明，供试大麦亲本材料可分为5个亚群。根据一般线性模型(general linear model, GLM)分析，共5个标记与大麦条纹病抗性性状关联，解释率在6.20%~11.15%之间，其中TACMD和MGB317达到极显著水平(P＜0.01)，解释率分别为9.24%和9.45%；根据混合线性模型(mixed linear model, MLM)分析，发现3个与大麦条纹病抗性相关的标记，解释率在4.57%~17.63%之间，标记HVM54达到极显著水平(P＜0.01)，解释率为17.63%。本研究为大麦条纹病抗病育种提供了一定理论依据。
Abstract：To understand the genetic diversity of barley(Hordeum vulgare) and identify SSR markers associated with resistance to barley leaf stripe, 100 pairs of SSR markers were used to detect the diversity of 86 barley entries. A total of 269 alleles were detected with an average alleles per locus of 2.69 and a range of 2~5. The allele frequency varied from 0.012 to 0.988, and the Shannon's index varied from 0.063 to 1.385. The genetic similarity ranged from 0.542 to 0.965. The polymorphism information content (PIC) value ranged from 0.023 to 0.737, with an average of 0.357. The barley entries were divided into 5 subgroups based on their population structure. Five markers were found to associate with barley leaf stripe resistance under general linear model(GLM)program, and the rate of phenotypic variation explained ranged from 6.20% to 11.15%. Two SSR markers, TACMD and MGB317, showed a significant association with resistance to barley leaf stripe (P＜0.01), which explained 9.24% and 9.45% of the phenotypic variation. Three markers were associated with resistance to barley leaf stripe under mixed linear model(MLM) programe, the rate of phenotype variation explained ranged from 4.57% to 17.63%. This study provides some theoretical basis to barley leaf stripe resistant breeding.
