The Construction of Dehydration-responsive Element (DRE) Reporter Yeast for the Screening of DRE-binding Transcription Factors From Tree Plant Populus simonii
Abstract：The expression of many induced genes is controlled by specific transcription factors and cis-acting element. Dehydration-responsive element (DRE) is very important in the regulation of the expression of drought and low-temperature responsive genes. DRE-binding transcription factors from Arabidopsis have been described. While research on DRE-binding transcription factors tree plants is scarce. Yeast one-hybrid approach is a powerful method for the searching for sequence-specific DNA binding transcription factors. Construction of DRE-containing report yeast strain is the first step for finding DRE-binding transcription factors from tree plants through yeast one-hybrid approach. This paper reports the construction of the DRE-containing report yeast strain. Our preliminary data show that the report strain we constructed is suitable for the screening for DRE-binding transcription factors from tree plants. Our work should be helpful for the improving of the stress-resistance features of tree plants.
韩素英 齐力旺 . 构建脱水反应元件（DRE）报告子以用于小叶杨DRE转录因子的克隆[J]. , 2007, 15(3): 0-.
. The Construction of Dehydration-responsive Element (DRE) Reporter Yeast for the Screening of DRE-binding Transcription Factors From Tree Plant Populus simonii. , 2007, 15(3): 0-.
