Cloning of cDNA Fragment of an Antennal-specific Gene in Helicoverpa armigera
Wang Guirong Guo Yuyuan Wu Kongming**
(State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)
Abstract：Abstract: The olfactory sensors of insects' antenna, which directly exposed to the environment, play an important role in identifying volatile hydrophobic molecules in the air. However, many odors in the air are cytotoxic xenobiotics, so a certain mechanism must exist in insect's antenna to decompose cytotoxic xenobiotics and redundant odorant stimulators. In the present study, differential display polymerase chain reaction (dd-PCR) was used to isolate three antennal -specific mRNA from the moth of cotton bollworm(Helicoverpa armigera ). Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins. Northern blot showed that A4-1 clone was specifically expressed in the antenna of H. armigera and the expressed level was higher in male antenna than that in female ones, which was similar to the expression of PBP-Harm gene from cotton bollworm. According to the gene's expression in insects' antenna and the role of glutathione S-transferase reported in other insects, the gene was predicted to be responsible for the decomposition of pheromone and harmful xenobiotics in antenna.
王桂荣 郭予元 吴孔明**. 一个棉铃虫触角特异表达基因cDNA片段的克隆[J]. , 2003, 11(1): 49-54.
Wang Guirong Guo Yuyuan Wu Kongming**. Cloning of cDNA Fragment of an Antennal-specific Gene in Helicoverpa armigera. , 2003, 11(1): 49-54.
