Abstract：Granulosa cells are the key factor affecting the development of ovarian follicles. miRNA affects the ovarian follicular development by controlling the proliferation and apoptosis of follicular granulosa cells. This study aimed to reveal the role of novel-miR-23900 in sheep (Ovis aries) follicular granulosa cells. First, isolated and cultured sheep follicular granulosa cells were treated with follicle-stimulating hormone (FSH), qPCR results showed that the expression levels of CDK2, CDK4, CCND2 and Bcl2 genes significantly increased (P<0.01), the Bax and Casp3 gene expression and novel-miR-23900 level significantly decreased (P<0.01). Then, novel-miR-23900 mimic and mimic negative control (NC) were further transfected into granulosa cells, CCK-8 was used to detect cell proliferation and mitochondrial membrane potential detection kit (JC-1) was used to detect cell apoptosis, and qPCR was used to detect the expression changes of genes related to proliferation and apoptosis. The results showed that the proliferation ability of granulosa cells significantly decreased at 72 and 96 h with transfection of novel-miR-23900 mimic (P<0.05), and extremely significantly decreased (P<0.01) at 48 h. The expression of proliferation-related genes CDK1, CDK4, CCNB1 and CCNB2 extremely significantly decreased (P<0.01), and the expression of CDK2, CCND1 and CCND2 significantly decreased (P<0.05). Apoptosis of granulosa cells was promoted by 48 h transfection with novel-miR-23900 mimic, the expression of apoptosis-related gene Bcl2 significantly decreased (P<0.01), the Casp3 gene expression significantly increased (P<0.01), and Bax gene expression significantly increased (P<0.05). In conclusion, novel-miR-23900 inhibited proliferation and promoted apoptosis in sheep granulosa cells. This study provides basic data for exploring the effects of novel-miR-23900 on sheep granulosa cells and its role in ovary.
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