Expression and Distribution of INHβB, FSHR and LHR in Yak (Bos grunniens) Antral Follicles
WANG Jun-Qian1, ZHAO Ling1, PAN Yang-Yang1, WANG Meng1, HUANG Zhen-Hua1, YANG Yi1, CUI Yan1, YU Si-Jiu1,2, WANG Li-Bin1,*
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Province Livestock Embryo Engineering Research Center, Lanzhou 730070, China
Abstract：Inhibin (INH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have important effects on the development of follicles. This study aimed to detect the expression of INHβB, FSH receptor (FSHR) and LH receptor (LHR) genes and proteins in yak (Bos grunniens) antral follicles with different diameters by qPCR and Western blot, and immunohistochemistry (IHC) was used to detect their distribution in yak antral follicles. The results showed that INHβB gene and protein expression in antral follicles increased first and then decreased with the increase of follicle diameter, and the highest expression level was found in large follicles (5~8 mm in diameter) (P<0.05). The FSHR gene and protein expression in large follicles (5~8 mm in diameter) were significantly higher than that in other follicles(P<0.05), while the expression of FSHR in other follicles with different diameters were not significantly different. LHR gene and protein expression increased with the increase of follicles diameter, and the highest expression level was found in the extra large follicles (diameter>8 mm)(P<0.05). IHC results showed that INHβB, FSHR and LHR mainly distributed in granulosa cells, cumulus cells and follicle membrane. The above results indicates that INHβB might has an important effect on follicle atresia or ovulation in yaks, and FSHR and LHR might enhance the effect of FSH and LH on follicle growth and development. This study provides a reference for improving the reproductive efficiency of yaks.
王军乾, 赵凌, 潘阳阳, 王萌, 黄振华, 杨贻, 崔燕, 余四九, 王立斌. INHβB、FSHR和LHR在牦牛有腔卵泡中的表达与分布[J]. 农业生物技术学报, 2023, 31(2): 334-340.
WANG Jun-Qian, ZHAO Ling, PAN Yang-Yang, WANG Meng, HUANG Zhen-Hua, YANG Yi, CUI Yan, YU Si-Jiu, WANG Li-Bin. Expression and Distribution of INHβB, FSHR and LHR in Yak (Bos grunniens) Antral Follicles. 农业生物技术学报, 2023, 31(2): 334-340.
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