Effects of Transport Stress on Tight Junction Proteins Claudin-1, Occludin and ZO-1 and Its Gene in Goat (Capra hircus) Small Intestine
WU Ya-Lin1*, YE Tian1*, FENG Ni1, YANG Yan-Zhen1, PENG Rui-Ni1, YANG Xiao-Xiao1, ZHENG Wen-Ya1,2,3*, ZHENG Lu-Cheng1, YANG Xue1
1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China;
2 Jiangxi Lvke Agriculture and Animal Husbandry Technology Co., Ltd., Yichun 336000, China;
3 Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, Yichun 336000, China
Abstract：There are many stresses during the rearing stage, but transport stress is unavoidable, such as capture, crowding, fasting are the most common transport stressors. These stressors can lead to reduced production performance of goat (Capra hircus) and even cause disease and huge economic losses. Since intestinal function is closely related to production performance, the changes of tight junction proteins related to intestinal barrier function under transport stress were explored. In order to investigate the effects of transport stress on tight junction proteins Claudin-1, Occludin and zonula occluden-1 (ZO-1) in goat small intestine, 18 goat were selected in this experiment and randomly divided into control group (transport 0 h group), 2 h transport group and 6 h transport group. The changes of Claudin-1, Occludin and ZO-1 genes and their protein expressions in goat small intestine were studied by immunohistochemistry and qPCR. Immunohistochemical results showed that Claudin-1, Occludin and ZO-1 proteins were mainly expressed in the cytoplasm of intestinal villi epithelial cells and intestinal gland epithelial cells of goat duodenum, jejunum and ileum. At the same time, the distribution of Claudin-1, Occludin and ZO-1 proteins changed among different groups in the same intestinal segment. The results of qPCR showed that compared with the control group, the expression levels of Claudin-1, Occludin and ZO-1 in the duodenum transit group for 2 and 6 h were significantly decreased (P<0.0001); The expression levels of Claudin-1, Occludin and ZO-1 in the transport 2 h group and 6 h group of jejunal were significantly increased (P<0.001); The expression levels of Claudin-1, Occludin and ZO-1 in the 6 h ileal transport group were significantly increased (P<0.01). It indicated that transport stress could affect the expression of Claudin-1, Occludin and ZO-1 genes and their proteins in goat small intestine. This study provides basic information to explore the mechanism of transportation stress and develop products related to transportation stress relief.
吴雅琳, 叶恬, 冯妮, 杨燕珍, 彭瑞妮, 杨敩校, 郑文亚, 郑路程, 杨雪. 运输应激对山羊小肠紧密连接蛋白 Claudin-1、Occludin 和 ZO-1 及其基因的影响[J]. 农业生物技术学报, 2022, 30(7): 1329-1338.
WU Ya-Lin, YE Tian, FENG Ni, YANG Yan-Zhen, PENG Rui-Ni, YANG Xiao-Xiao, ZHENG Wen-Ya,,ZHENG Lu-Cheng, YANG Xue. Effects of Transport Stress on Tight Junction Proteins Claudin-1, Occludin and ZO-1 and Its Gene in Goat (Capra hircus) Small Intestine. 农业生物技术学报, 2022, 30(7): 1329-1338.
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