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2025年4月3日 星期四
农业生物技术学报  2022, Vol. 30 Issue (5): 918-925    DOI: 10.3969/j.issn.1674-7968.2022.05.009
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
干扰CIDEa基因对奶山羊乳腺上皮细胞脂质合成的影响
李君1,2, 孙雨婷3, 郭震楠1, 韩浩园1, 赵金艳1, 徐秋良1, 牛晖1, 权凯1,*
1 河南牧业经济学院 动物科技学院,郑州 450046;
2 河南省反刍动物营养与饲料资源开发国际联合实验室,郑州 450046;
3 陕西省动物研究 所,西安 710032
Effect of CIDEa Interference on Lipid Synthesis of Mammary Epithelial Cells in Dairy Goats (Capra hircus)
LI Jun1,2, SUN Yu-Ting3, GUO Zhen-Nan1, HAN Hao-Yuan1, ZHAO Jin-Yan1, XU Qiu-Liang1, NIU Hui, QUAN Kai1,*
1 College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2 International Joint Laboratory of Ruminant Nutrition and Feed Resources Development in Henan Province, Zhengzhou 450046, China;
3 Shaanxi Institute of Zoology, Xi'an 710032, China
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摘要 诱导细胞凋亡 DFF45 样效应子 a (cell death inducing DNA fragmentation factor 45 like effector a, CIDEa)是一类和脂类代谢紧密相关的蛋白,参与调控脂解、脂滴的生长融合和极低密度脂蛋白的成熟。 为研究 CIDEa 基因在奶山羊(Capra hircus)乳腺上皮细胞中的生物学功能 ,本研究利用 qPCR 技术检测CIDEa 在奶山羊泌乳期和干奶期乳腺组织的表达差异;采用 siRNA 介导的干扰技术研究 CIDEa 基因对脂 质合成和相关基因表达的影响。结果发现,CIDEa 基因在泌乳期乳腺组织的表达量显著高于干奶期(P< 0.05)。利用 siRNA 介导的干扰技术,在奶山羊乳腺上皮细胞中转染 siRNA,CIDEa 基因 mRNA 水平下调60%~88% (P<0.01);干扰 CIDEa,抑制了细胞中脂滴积累 ,显著降低细胞中甘油三酯含量(P<0.05);CIDEa 干扰后,显著上调脂肪酸合酶(fatty acid synthase, FASN)和激素敏感脂酶(hormone-sensitive lipase, HSL)基因的表达(P<0.05),同时促进固醇调节元件结合蛋白 1 (sterol regulatory element binding proteins 1, SREBP1)基因的表达(P<0.05),并且抑制脂肪酸结合蛋白 3 (fatty acid-binding protein 3, FABP3)和二酰 基 甘 油 酰 基 转 移 酶 1(diacylglycerol acyltransferase 1, DGAT1) 基 因 的 表 达 (P<0.05)。研 究 结 果表 明 , CIDEa 基因在奶山羊乳腺上皮细胞中促进脂滴合成,本研究为进一步研究CIDEa调控奶山羊乳脂合成的作用机制提供理论依据。
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李君
孙雨婷
郭震楠
韩浩园
赵金艳
徐秋良
牛晖
权凯
关键词 CIDEa 基因乳腺上皮细胞siRNA脂质合成甘油三酯    
Abstract:Cell death inducing DNA fragmentation factor 45 like effector a (CIDEa) is a kind of protein closely related to lipid metabolism. It is involved in the regulation of lipolysis, the growth and fusion of lipid droplets and the maturation of very low density lipoprotein. In order to determine the biological function of CIDEa in dairy goat mammary gland, the mRNA level of CIDEa in mammary gland was detected using qPCR during lactation and dry period. Then, siRNA mediated interference technique was used to study the effect of CIDEa on lipid synthesis and the expression of related genes. The results showed that the expression of CIDEa mRNA in mammary gland was higher in lactation than that in dry period (P<0.05). The siRNAs targeting CIDEa gene were transfected into dairy goat mammary epithelial cells, and the mRNA level of CIDEa was inhibited by 60%~88% (P<0.01). The accumulation of lipid droplets and the content of triglyceride in cells were inhibited after interfering CIDEa expression. In addition, interfering CIDEa expression significantly upregulated the expression of hormone sensitive lipase (HSL) and fatty acid synthase (FASN) (P<0.05), and also promoted the expression of sterol regulatory element binding protein 1 (SREBP1) (P<0.05). Furthermore, the expression of fatty acid binding protein 3 (FABP3) and diacylglycerol transferase gene 1 (DGAT1) (P<0.05) were inhibited when interfering CIDEa gene expression. The results showed that CIDEa promoted the synthesis of lipid droplets in dairy goat mammary epithelial cells, which provides a theoretical basis for further study the molecular mechanism of CIDEa gene regulating milk lipid synthesis in dairy goat.
Key wordsCIDEa gene    Mammary epithelial cells    siRNA    Lipid synthesis    Triglyceride
收稿日期: 2021-07-31     
ZTFLH:  S827  
基金资助:国家自然科学基金(31601915); 河南牧业经济学院科研创新基金(XKYCXJJ2020013)
通讯作者: * quankai1115@163.com   
引用本文:   
李君, 孙雨婷, 郭震楠, 韩浩园, 赵金艳, 徐秋良, 牛晖, 权凯. 干扰CIDEa基因对奶山羊乳腺上皮细胞脂质合成的影响[J]. 农业生物技术学报, 2022, 30(5): 918-925.
LI Jun, SUN Yu-Ting, GUO Zhen-Nan, HAN Hao-Yuan, ZHAO Jin-Yan, XU Qiu-Liang, NIU Hui, QUAN Kai. Effect of CIDEa Interference on Lipid Synthesis of Mammary Epithelial Cells in Dairy Goats (Capra hircus). 农业生物技术学报, 2022, 30(5): 918-925.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.05.009     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I5/918
 
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