Identification of FAF Gene Family and Excavation of Branch Forming Related Members in Brassica napus
JIANG Xian-Run1, LUO Gui-Lin1, LIANG Feng-Hao2, HUANG Sha2, XIAO Hua-Gui2, ZHANG Chao2,*, DAI Wen-Dong3,*
1 College of Agriculture, Guizhou University, Guiyang 550025, China; 2 Guizhou Oil Crops Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China; 3 Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, China
Abstract：The FAF gene family is one of the unique transcription factors in the plant kingdom and plays a role in regulating the size of meristem. In order to study the effect of FAF gene on branch formation in Brassica napus, genome-wide identification and bioinformatics analysis of FAF family in B. napus were performed in this study. In addition, qPCR was used to investigate the spatiotemporal expression of BnFAFs in the 3 pairs of extreme phenotype materials with different branch number. The results showed that there were 17 BnFAFs distributing on 13 chromosomes in B. napus. Based on the similarity of amino acid sequence, BnFAFs were divided into 4 subfamilies Ⅰ ~ Ⅳ by referring to Arabidopsis FAFs. Gene structure and protein structure identification showed that members of Ⅳ subfamilies were rather differentiated, while other subfamily members were relatively conserved. qPCR analysis showed that the expression pattern of BnFAF4-4 and BnFAF4-7 were consistent, and the relative expression of BnFAF4-4 and BnFAF4-7 in stem, upper axillary bud and middle axillary bud of multi-branched materials were significantly higher than that of less branched materials in bolting stage suggesting that these genes may affect the formation of branches. This study laid a foundation for further improvement of branch traits by using FAF gene in B. napus.
蒋先润, 罗桂林, 梁峰豪, 黄莎, 肖华贵, 张超, 代文东. 甘蓝型油菜FAF基因家族鉴定并挖掘分枝形成相关成员[J]. 农业生物技术学报, 2022, 30(5): 847-860.
JIANG Xian-Run, LUO Gui-Lin, LIANG Feng-Hao, HUANG Sha, XIAO Hua-Gui, ZHANG Chao, DAI Wen-Dong. Identification of FAF Gene Family and Excavation of Branch Forming Related Members in Brassica napus. 农业生物技术学报, 2022, 30(5): 847-860.
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