Application and Prospect of Nanopore Sequencing Technology in Plant Pathogen Detection
LU Hui-Xin, SUN Kai*, YIN Chuan-Lin, HUANG Yu-Dong, YU Xiao-Ping*
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University, Hangzhou 310018, China
Abstract：Plant pathogenic agents seriously threaten the yield and quality of crop. Establishing efficient and accurate pathogen diagnosis technology is critical for disease control. Metagenomic sequencing technology based on next generation sequencing (NGS) platform is an important tool for plant disease identification. However, the application of NGS in the rapid detection of plant pathogens is restricted by the disadvantages of high cost, long experimental period and bulky equipment. Nanopore sequencing technology developed by Oxford Nanopore Technology Co., Ltd. (ONT)(Oxford, United Kingdom) is a new sequencing platform. Compared with the NGS platform, it has advantages such as low cost, short experimental period and easy to use. It is suitable for field detection and has been used in pathogen detection. In this paper, the principle of ONT Nanopore sequencing technology was introduced, and the applications and challenges of this technology in plant pathogen detection were summarized, which provides reference for the further application and improvement of this technology in plant pathogen detection.
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