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2025年8月14日 星期四
农业生物技术学报  2021, Vol. 29 Issue (6): 1040-1049    DOI: 10.3969/j.issn.1674-7968.2021.06.002
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
番茄成熟过程中细胞壁代谢相关基因的表达及外源乙烯和ABA处理对其表达的影响
曾文静, 杨军, 陈世美杰, 韦小英, 文国琴, 何卓远, 王倩, 陈奚潼, 周家燕, 邹建*
西华师范大学 西南野生动植物资源保护省部共建(教育部)重点实验室,南充 637009
Expression of Cell Wall Metabolism Genes During Tomato (Solanum lycopersicum) Maturation and the Effects of Treatment with Exogenous C2H4 and ABA on Their Expression
ZENG Wen-Jing, YANG Jun, CHEN Shi-Mei-Jie, WEI Xiao-Ying, WEN Guo-Qin, HE Zhuo-Yuan, WANG Qian, CHEN Xi-Tong, ZHOU Jia-Yan, ZOU Jian*
Key laboratory of Southwest Wildlife Resources Protection (Ministry of Education), China West Normal University, Nanchong 637009, China
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摘要 果实硬度是衡量其货架期的一个重要指标,细胞壁代谢是影响果实硬度及其软化进程的重要因素。为解析番茄软化进程与细胞壁代谢基因和促成熟激素调控之间的关系,本研究通过施加外源激素及其抑制剂,并利用qRT-PCR技术系统分析了番茄(Solanum lycopersicum)成熟过程中细胞壁代谢基因的表达变化,研究了促成熟激素脱落酸(abscisic acid, ABA)和乙烯(C2H4)及其抑制剂对这些基因表达的影响。结果表明,随着果实的成熟和软化,细胞壁代谢相关基因SlCel1 (endo-β-1, 4-glucanases)、SlTBG7 (β-galactosidase precursor)、SlXETB2-F2 (xyloglucanendotransglycosylase)、SlXET4-F1SlTBG3SlCel2SlExp1 (expansin)、SlTBG1SlPG (polygalacturonase)和SlTBG4的表达量增加,说明这些细胞壁代谢基因与番茄成熟过程中的软化进程密切相关。外源ABA和乙烯及其抑制剂NDGA (nordihydroguaiaretic acid)或1-MCP (1-methylcyclopropene)处理的结果显示,ABA处理促使SlCel1SlExp1SlTBG3SlTBG4SlXET4-F1基因表达上调,而SlCel1SlExp1SlTBG4基因受到NDGA抑制。另外,施加乙烯能使SlExp1SlPGSlTBG4基因表达量增加,其表达受1-MCP抑制。上述结果说明SlCel1SlExp1SlTBG3SlTBG4SlPGSlXET4-F1等细胞壁代谢基因参与果实成熟过程中的软化进程,其表达可能受ABA和乙烯的调控。因此,合理施用NDGA或1-MCP有助于抑制细胞壁代谢,延长果实的货架期。而果实成熟过程中SlPGSlCel1SlExp1SlTBG4基因低水平表达的番茄新品种的培育可能成为番茄采后保鲜、保持果实良好品质的新途径,这对实现番茄果实的成熟调控有指导意义。
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曾文静
杨军
陈世美杰
韦小英
文国琴
何卓远
王倩
陈奚潼
周家燕
邹建
关键词 番茄果实硬度细胞壁代谢基因促成熟激素促成熟激素抑制剂    
Abstract:Firmness is an important indicator of fruit shelf-life, and cell wall metabolism is an important factor affecting the firmness and softening process of fruit. In order to understand the relationship among the softening process, cell wall metabolism genes and regulation of fruit ripening hormones in tomato (Solanum lycopersicum), the expression of cell wall metabolism genes during tomato fruit ripening was systematically analyzed, and the effects of the fruit ripening hormones, abscisic acid (ABA), ethylene (C2H4) and their inhibitors on the expression of these genes were investigated by applying exogenous hormones and their inhibitors and qRT-PCR technique. The results showed that the expression of cell wall metabolism related genes, SlCel1 (endo-β-1,4-glucanases), SlTBG7 (β-galactosidase precursor), SlXETB2-F2 (xyloglucanendotransglycosylase), SlXET4-F1, SlTBG3, SlCel2, SlExp1 (expansin), SlTBG1, SlPG (polygalacturonase) and SlTBG4 are increased with fruit maturation and softening, suggesting their close relation to the softening process during fruit ripening in tomato. The treatment results of fruit ripening hormones and their inhibitors indicated that exogenous ABA could promote the expressing of SlCel1, SlExp1, SlTBG3, SlTBG4 and SlXET4-F1 genes,while the expression of SlCel1, SlExp1 and SlTBG4 genes was inhibited by NDGA (nordihydroguaiaretic acid). The application of ethylene could up-regulate the expressing of SlExp1, SlPG and SlTBG4 genes, but their expressions were inhibited by 1-MCP (1-methylcyclopropene). These results implied that the regulation of cell wall metabolism related genes, SlCel1, SlExp1, SlTBG3, SlTBG4, SlPG and SlXET4-F1 require ABA and ethylene to regulate the softening process during fruit ripening. Therefore, appropriate application of NDGA or 1-MCP might be beneficial for inhibiting cell wall metabolism and would extend the fruit shelf-life by inhibiting the ABA and ethylene signaling. The cultivation of new tomato varieties with low expression levels of SlPG, SlCel1, SlExp1 and SlTBG4 genes may effectively realize a new way to keep tomatoes fresh and maintain good fruit quality after harvest. These results has guiding significance for realizing the ripening regulation of tomato fruit.
Key wordsSolanum lycopersicum    Fruit firmness    Cell wall metabolism gene    Maturation hormone    Maturation hormone inhibitor
收稿日期: 2020-09-14     
ZTFLH:  S371  
基金资助:国家自然科学基金(31171587); 西华师范大学2018年博士科研启动专项(18Q039); 西华师范大学英才基金(17YC354)
通讯作者: *zoujian@cwnu.edu.cn   
引用本文:   
曾文静, 杨军, 陈世美杰, 韦小英, 文国琴, 何卓远, 王倩, 陈奚潼, 周家燕, 邹建. 番茄成熟过程中细胞壁代谢相关基因的表达及外源乙烯和ABA处理对其表达的影响[J]. 农业生物技术学报, 2021, 29(6): 1040-1049.
ZENG Wen-Jing, YANG Jun, CHEN Shi-Mei-Jie, WEI Xiao-Ying, WEN Guo-Qin, HE Zhuo-Yuan, WANG Qian, CHEN Xi-Tong, ZHOU Jia-Yan, ZOU Jian. Expression of Cell Wall Metabolism Genes During Tomato (Solanum lycopersicum) Maturation and the Effects of Treatment with Exogenous C2H4 and ABA on Their Expression. 农业生物技术学报, 2021, 29(6): 1040-1049.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.06.002     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I6/1040
 
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