Screening, Identification and Biological Characteristics of An Enzyme-producing Bacillus Strain Isolated from Siniperca chuatsi
WANG Jia-Chuan1,2, ZHANG De-Feng2, WANG Ya-Jun2,*, LIANG Chi-Qiang3, XIONG Xiu-Ling2, SHI Cun-Bin2,*, WANG Fang2, LIU Li-Juan1,2
1 Aquaculture Institute, Tianjin Agricultural University, Tianjin 300384, China;
2 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Drug Development, Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou 510380, China;
3 Integrated Agricultural Service Center of Qingcheng District, Qingyuan 511500, China
Abstract：Mandarin fish (Siniperca chuatsi) has low utilization rate of artificial feed and high breeding cost. In present study, in order to improve the palatability and utilization rate of artificial feed for mandarin fish, Bacillus spp. with enzyme producing ability was screened from the intestine of healthy mandarin fish. The intact intestines of 16 healthy mandarin fish were selected aseptically, and the homogenate was diluted in a stepwise manner to screen the strains producing amylase, protease and cellulase by the plate coating method. After comprehensively analyzing the enzyme-producing ability of the strains, a strain GY65 with strong enzyme-producing ability was selected. The strain GY65 was determined to be Bacillus velezensis according to morphological observation, physiological and biochemical characteristics analysis and genes evolution analysis of 16S rRNA and gyrA. The growth characteristics showed that the optimal pH of strain GY65 was 7, and the growth of strain GY65 was promoted by the low concentration of Mg2+, Ca2+, Mn2+ and Fe3+. Biosafety test showed that the bacteria was sensitive to most antibiotics; and non-pathogenic to mandarin fish, snakehead (Channa argus), nile tilapia (Oreochromis niloticus) and zebra fish (Danio rerio). In conclusion, the strain GY65 has good biological safety and the potential as a feed additive for mandarin fish. This study provides a reference for improving the utilization rate of artificial feed for mandarin fish.
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基金项目:国家肉牛牦牛产业技术体系专项(CARS-37);河南省肉牛产业技术体系; S201910712062; X201910712408)
