Genetic Diversity of g.-38T>C and g.509A>G in ANGPTL3 and Their Association Analysis with Growth and Carcass Traits in Qinchuan Cattle (Bos taurus)
LI Ya-Xing1, *, CHENG Gong2, *, ZHAO Yun-Bo1, WANG Shen-Yuan3, WU Kai-Feng3, CHENG Li-Xin4
1 State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock/College of Life Sciences, Inner Mongolia University, Hohhot 010071, China; 2 National Beef Cattle Improvement Center/College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 3 Inner Mongolia Key Laboratory of Biomanufacture/College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010031, China; 4 Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry, Hohhot 010031, China; 5 Key Laboratory of Animal Genetics, Breeding, and Reproduction/National Engineering Laboratory for Animal Breeding, Ministry of Agriculture/College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Abstract：The growth rate and carcass quality of beef cattle (Bos taurus) have important economic value in beef cattle industry. Angiopoietin-like protein 3 gene (ANGPTL3) is known to be a kind of secreted protein, and plays an important role in lipid, glucose and energy metabolisms. A previous study reported that there were g.-38T>C and g.509A>G mutations identified in ANGPTL3 gene, which were associated with growth and carcass traits in beef cattle. The aims of this study were to investigate the genetic diversity of g.-38T>C and g.509A>G mutations in different cattle populations, and confirm their association with growth and carcass traits in Qinchuan cattle (B. taurus). Total of 599 cattle sample (including 384 Qinchuan cattle, 24 Luxi cattle, 41 Mongolia cattle from Inner Mongolia, 50 Mongolia cattle from Mongolia, 50 Wuling cattle and 50 Longlin cattle) were collected for this study. All of 599 samples were genotyped by direct sequencing and MassARRAY technologies, and then the analyses of genetic diversity in 6 cattle populations and association with growth and carcass traits in Qinchuan cattle were performed. The results showed that the genotype frequency of g.-38T>C and g.509A>G in ANGPTL3 were consistent with Hardy-Weinberg equilibrium (P＜0.05) in each of 6 cattle populations. There were 3 genotypes TT (0.695), TC (0.261) and CC (0.044) of g.-38T>C mutation in Qinchuan cattle population. Association analysis results showed that g.-38T>C SNP was significantly associated with body length, hip width, chest circumference and back fat thickness (P＜0.05), as well as ultrasound loin muscle area and intramuscular fat content (P＜0.01) in Qinchuan cattle. Furthermore, there were 3 genotypes AA (0.690), AG (0.279) and GG (0.031) of g.509A>G mutation in Qinchuan cattle population. Association analysis results showed that g.509A>G SNP was significantly associated with body length, pin bone width, hip width and intramuscular fat content (P＜0.05), as well as chest circumference and ultrasound loin muscle area (P＜0.01). The combination analysis of two SNPs showed that the individuals with TC and AG genotypes had significantly greater ultrasound loin muscle area and intramuscular fat content than individuals with TT and AA (P＜0.05). Thus, these results suggested that g.-38T>C and g.509A>G in ANGPTL3 gene could be an effective molecular marker for marker-assisted breeding of Qinchuan cattle, and these results could provide scientific basis for genetic improvement of Qinchuan cattle.
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