Study on the Function of Arginine Decarboxylase Gene TaADC in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina
LI Tong-Tong2, SONG Shan-Shan2, WANG Qi2, HOU Chun-Yan1,2,3, WANG Dong-Mei1,2,3,*
1 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China;
2 College of Life Sciences, Hebei Agricultural University, Baoding 071000, China;
3 Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, China
Abstract：Arginine decarboxylase (ADC) is a key enzyme in the synthesis of polyamines, which plays an important role in the resistance of plants to stress. In the early stage of this study, by analyzing the transcriptome database of wheat (Triticum aestivum) and leaf rust fungus (Puccinia triticina) interaction, it was screend that the expression of TaADC in the compatible and incompatible combinations was signifigant difference. On this basis, qRT-PCR detection showed that the expression of TaADC was induced by Puccinia triticina infection in the incompatible combination, not induced in the compatible combination. Transient transformation of recombinant plasmid 35S::TaADC-GFP into tobacco (Nicotiana tabacum) revealed that TaADC localized in the cytoplasm. Virus-induced gene silencing (VIGS) and RNA interference (RNAi) techniques were used to silence TaADC gene. Compared with TaADC non-silent plants (control), silent plants showed increased number of haustorium mother cells (HMC) and area of host hypersentive reaction (HR) in single infection site. The above results proved that TaADC might play an important role in the occurrence of HR in wheat resistance to Puccinia triticina infection. This study might provide new evidence for elucidating the molecular mechanism of HR in the interaction between wheat and Puccinia triticina.
李彤彤, 宋姗姗, 王琦, 侯春燕, 王冬梅. 小麦与叶锈菌互作过程中精氨酸脱羧酶基因TaADC的功能研究[J]. 农业生物技术学报, 2020, 28(8): 1343-1351.
LI Tong-Tong, SONG Shan-Shan, WANG Qi, HOU Chun-Yan, WANG Dong-Mei. Study on the Function of Arginine Decarboxylase Gene TaADC in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina. 农业生物技术学报, 2020, 28(8): 1343-1351.
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