Amplification and Analysis of ITS Sequence of Intestinal Parasites in Przewalski's Wild Horse (Equus ferus ssp. przewalskii)
LI Qiao1, ZHAO Jian-You3, DU Xiao-Hua2, LU Wei3, ZHANG Chun-Lin3, DONG Jian-Ying2, WEN Yong-Qiang1, ZHOU Ying-Jie3,*, LIU Xia1,*
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 3 State Forest Administration Gansu Protection Centre of Threatened Species, Wuwei 733000, China
Abstract：Przewalski's wild horse (Equus ferus ssp. przewalskii) is a subspecies of E. ferus and is considered as endangered animals. The healthy growth and continuous expansion in their population have special value for germplasm resource protection. The parasites in Przewalski's wild horse can produce different degrees of pathological damage to the organism. The present study aimed to identify the intestinal parasite species in Przewalski's wild horses which were taken from the State Forest Administration Gansu Protection Centre of Threatened Species. During sampling procedures, the worms were separated in the faeces of Przewalski's wild horses. Morphological characteristics were identified and ITS (internal transcribed spacer) sequences were compared using bioinformatic methods. The results showed that 2 kinds of worms named PE and CN were isolated from intestinal tracts of Przewalski's wild horses, and preliminarily identified as Parascaris equorum and Cylicocyclus sp. by morphology observation. The full length of PE ITS region was 865 bp (GenBank No. MT579850), and the length of ITS-1, 5.8S rDNA and ITS-2 was 421, 156 and 288 bp, respectively; and the homology with Parascaris equorum was 99.88%. The full length of CN ITS region was 843 bp (GenBank No. MT581488), and the length of ITS-1, 5.8S rDNA and ITS-2 was 370, 153 and 320 bp, respectively; and the homology with C. nassatus was 99.41%. The above results showed that PE sample collected from Przewalski's wild horses was Parascaris equorum and the CN sample was C. nassatus. The results of this study could provide a reference for healthy breeding of Przewalski's wild horses.
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