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2025年8月30日 星期六
农业生物技术学报  2019, Vol. 27 Issue (6): 1072-1080    DOI: 10.3969/j.issn.1674-7968.2019.06.013
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
草鱼PKMa基因SNPs筛选及与耐糖性状的关联分析
樊佳佳, 唐小红, 白俊杰, 姜鹏*, 马冬梅, 李胜杰
中国水产科学研究院 珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点试验室,广州 510380
Screening of SNPs in PKMa Gene and Analysis of Its Correlations with Glucose Tolerance Trait in Ctenopharyngodon idella
FAN Jia-Jia, TANG Xiao-Hong, BAI Jun-Jie, JIANG Peng*, MA Dong-Mei, LI Sheng-Jie
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture/Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
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摘要 丙酮酸激酶(pyruvate kinase, PK)是糖酵解途径中最后一个关键限速酶,在动物糖代谢过程中发挥重要作用。研究发现,草鱼(Ctenopharyngodon idella)Ma型丙酮酸激酶基因(Ma-type pyruvate kinase, PKMa)的3'非编码区存在2个SNPs位点:T+1817C和G+1818T;两位点连锁组成3种单倍型:TG/TG、TG/CT和CT/CT(分别简称: AA, AB和BB)。为探究草鱼PKMa基因多态性对机体耐糖能力的影响,本研究设计低糖(low-glucose, LD)和高糖(high-glucose, HD)两种饲料养殖环境,比较3种PKMa基因型个体在不同糖水平下的生长性能。8周饲养实验结果显示:LD组草鱼生长速度快于HD组,其中LD组AA单倍型个体体质量分别比AB、BB单倍型增加4.9%和6.1%(P>0.05);HD组AA单倍型个体体质量分别比AB、BB型增加36.8%和30.4%(P<0.05),且比LD组AA单倍型增加6.7%。针对PKMa基因在脑组织特异表达,检测脑组织PK酶浓度,发现LD组PK酶浓度低于HD组;两组中AA单倍型样本PK浓度均高于AB和BB单倍型。研究结果表明,PKMa基因多态性与草鱼耐糖能力存在关联。饲料糖水平过高对草鱼生长有一定程度的抑制,但AA单倍型个体表现出较好的耐受性,推测该突变有助于提高草鱼PK酶的表达,从而更好地利用饲料中的糖源。该研究可为耐糖草鱼新品种(系)的选育提供候选分子标记。
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樊佳佳
唐小红
白俊杰
姜鹏
马冬梅
李胜杰
关键词 草鱼丙酮酸激酶SNP单倍型耐糖    
Abstract:Pyruvate kinase (PK) is a key rate-limiting enzyme that catalyzes the last step of glycolysis and plays an important role in animal glucose metabolism. Studies have shown that there were 2 SNPs (T+1817C and G+1818T) in the 3' non-coding region of Ma-type pyruvate kinase (PKMa) gene of Ctenopharyngodon idella. The completely linked SNPs were combined into 3 genotypes, TG/TG, CT/TG and CT/CT (abbreviated as AA, AB and BB), respectively. In order to investigate the PKMa gene polymorphism effect on glucose tolerance, the growth performance of the 3 genotypes were tested and compared in the low-glucose (LD) and high-glucose (HD) diet environments. After 8 weeks feeding trial, the results showed that the growth rate of grass carp in LD group was faster than that in HD group. In the LD population, the body weight of AA haplotype was 4.9% and 6.1% faster than AB haplotype and BB haplotype, respectively (P>0.05). In the HD population, the body weight of the AA haplotype was 36.8% and 30.4% faster than the AB haplotype and the BB haplotype, respectively (P<0.05) and increased by 6.7% compared with LD population AA haplotype. For the specific expression of PKMa gene in brain tissue, the concentration of PK enzyme in brain tissue was detected. It was shown that the PK enzyme concentration in LD population was lower than that in HD population. The AA genotype individuals was significantly higher than that of the AB and BB haplotypes individuals in the LD and HD population, respectively. The results showed that the PMKa gene mutation was associated with the body's tolerance to glucose of C. idella. It is speculated that the expression level of the PKMa enzyme concentration is increased to better utilize the glucose dietary having the AA genotype C. idella. This study provides candidate markers for the breeding of new varieties of glucose tolerant in C. idella.
Key wordsCtenopharyngodon idella    Pyruvate kinase    SNP    Haplotype    Glucose tolerance
收稿日期: 2019-01-18     
ZTFLH:  S917.4  
基金资助:中国水产科学研究院基本科研业务费(No. 2018SJ-YZ03);国家大宗淡水鱼产业技术体系建设(No. CARS-45-04);广东省自然科学基金(No. 2016A030313148)
通讯作者: jiangpeng85@126.com   
引用本文:   
樊佳佳, 唐小红, 白俊杰, 姜鹏, 马冬梅, 李胜杰. 草鱼PKMa基因SNPs筛选及与耐糖性状的关联分析[J]. 农业生物技术学报, 2019, 27(6): 1072-1080.
FAN Jia-Jia, TANG Xiao-Hong, BAI Jun-Jie, JIANG Peng, MA Dong-Mei, LI Sheng-Jie. Screening of SNPs in PKMa Gene and Analysis of Its Correlations with Glucose Tolerance Trait in Ctenopharyngodon idella. 农业生物技术学报, 2019, 27(6): 1072-1080.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.06.013     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I6/1072
 
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