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农业生物技术学报  2019, Vol. 27 Issue (5): 799-808    DOI: 10.3969/j.issn.1674-7968.2019.05.004
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
无乳链球菌感染对吉富罗非鱼行为的影响及其机制
衣萌萌, 王淼, 李忠徽, 张德锋, 刘志刚, 高风英, 卢迈新*
中国水产科学研究院 珠江水产研究所/农业农村部热带亚热带水产资源利用与养殖重点实验室,广州 510380
The Impacts and Mechanism of Streptococcus agalactiae on Behavior of GIFT Tilapia (Oreochromis niloticus)
YI Meng-Meng, WANG Miao, LI Zhong-Hui, ZHANG De-Feng, LIU Zhi-Gang, Gao Feng-Ying, LU Mai-Xin*
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture/Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China
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摘要 在罗非鱼养殖过程中,无乳链球菌(Streptococcus agalactiae)感染会导致罗非鱼行为异常,并严重危害罗非鱼产业的健康发展。为了研究无乳链球菌感染后吉富罗非鱼(Oreochromis niloticus)行为变化的生理机制,本研究采用qRT-PCR和免疫荧光的方法,以人工腹腔注射感染无乳链球菌的吉富罗非鱼为研究对象,检测感染前后吉富罗非鱼脑组织和肠道组织中无乳链球菌含量、行为变化以及5-羟色胺(5-hydroxytryptamine, 5-HT)在其小脑、肠道和胃中的表达情况。结果表明,吉富罗非鱼脑组织和肠道组织中无乳链球菌的含量分别从人工感染后的第6和第0小时开始急剧增加,均在第18小时达到高峰,其中脑组织峰值61.2 copies/mg,肠道组织峰值1 800.5 copies/mg;实验鱼在人工感染后的第12小时出现无定向乱窜、持续旋转游动的异常行为,并且这种异常行为一直持续到实验结束或实验鱼死亡;在人工感染前和感染后的实验鱼小脑、肠道和胃中均发现5-HT阳性反应,但是小脑和肠道中5-HT的阳性反应随着病原感染时间延长呈现变弱的趋势,在实验第18小时时荧光强度分别为9.1和6.9,而胃组织中5-HT阳性反应随着病原感染时间延长呈现先升高后降低的趋势,在实验末期到达最低值3.1。因此可以推测,无乳链球菌感染罗非鱼后,通过调控脑中枢神经系统和外周消化道系统中的5-HT使罗非鱼产生无定向乱窜和持续旋转的异常行为。本研究从5-HT的角度解释无乳链球菌感染后罗非鱼的异常行为,可为研究感染病原后鱼类冲动行为的机制提供新的研究思路。
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衣萌萌
王淼
李忠徽
张德锋
刘志刚
高风英
卢迈新
关键词 无乳链球菌罗非鱼行为5-HT    
AbstractStreptococcus agalactiae infection may cause abnormal behavior of tilapia (Oreochromis niloticus) and seriously endangers the development of the tilapia industry.To investigate the impacts of S.agalactiae on behavior of GIFT tilapia and its mechanism, the qRT-PCR and immunofluorescence were used to examine the concentration of S.agalactiae in the brain and intestine, the behavior changes, as well as the expression of 5-hydroxytryptamine (5-HT) in the cerebellum, intestine and stomach of tilapia infected with by intraperitoneal injection.The results showed that the copies of S.agalactiae in the brain and intestinal tissues of tilapia increased sharply from the 6th and 0 h after pathogen infection respectively, and reached the maximum at 18th h (61.2 copies /mg in the brain and 1 800.5 copies/ mg in the intestine).Furthermore, fish showed abnormal behavior from the 12th h after pathogen challenge, and the abnormal behavior continued until the end of the experiment or the death of the fish.In addition, 5-HT positive reaction was found in the cerebellum, intestine and stomach of tilapia before and after S. agalactiae infection.While the positive reaction of 5-HT in cerebellum and intestine showed a weakening trend with the prolongation of experiment, and the fluorescence intensity of cerebellum and intestine were 9.1 and 6.9 at 18th h, respectively.But the 5-HT positive reaction in stomach increased first and then decreased with experiment went on, reaching a minimum of 3.1 at the end of the experiment.Therefore, it could be speculated that after infection of S.agalactia, tilapia induces abnormal behavior of tilapia by regulating 5-HT in the central nervous system and the peripheral digestive system.The current study researched the abnormal behavior of tilapia after S.agalactiae infection from the perspective of 5-HT, which could provide a new insight into the mechanism of impulsive behavior of fish infected with pathogen.
Key wordsStreptococcus agalactiae    Behavior of tilapia    5-HT
收稿日期: 2018-11-23     
ZTFLH:  S943  
基金资助:中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(No.2016HY-ZD1403)、现代农业产业技术体系专项资金(CARS-46)和广州市科技计划项目(No.201707010312)
通讯作者: mx-lu@163.com   
引用本文:   
衣萌萌, 王淼, 李忠徽, 张德锋, 刘志刚, 高风英, 卢迈新. 无乳链球菌感染对吉富罗非鱼行为的影响及其机制[J]. 农业生物技术学报, 2019, 27(5): 799-808.
YI Meng-Meng, WANG Miao, LI Zhong-Hui, ZHANG De-Feng, LIU Zhi-Gang, Gao Feng-Ying, LU Mai-Xin. The Impacts and Mechanism of Streptococcus agalactiae on Behavior of GIFT Tilapia (Oreochromis niloticus). 农业生物技术学报, 2019, 27(5): 799-808.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.05.004     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I5/799
 
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