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2025年4月5日 星期六
农业生物技术学报  2019, Vol. 27 Issue (3): 526-533    DOI: 10.3969/j.issn.1674-7968.2019.03.017
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
解淀粉芽胞杆菌对玉米秸秆的降解特性
杨娟, 王艳, 蔡云花, 李红亚*, 李术娜, 王树香, 王全
河北农业大学 生命科学学院,保定071001
Degradation Characteristics of Corn (Zea mays) Straw by Bacillus amyloliquefaciens
YANG Juan, WANG Yan, CAI Yun-Hua, LI Hong-Ya*, LI Shu-Na, WANG Shu-Xiang, WANG Quan
College of Life Science, Hebei Agricultural University, Heibei Baoding, 071000, China
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摘要 利用微生物发酵技术降解玉米(Zea mays)秸秆,对于实现纤维素类生物质的资源化利用意义重大。本研究在课题组前期获得1株可降解纤维素的解淀粉芽胞杆菌(Bacillus amyloliquefaciens)MN-13菌株的基础上,进一步考察了该菌株的纤维素酶活力以及对玉米秸秆纤维素的降解特性,通过气质联用色谱对秸秆纤维素类成分的降解产物进行了分析,旨在为菌株MN-13发酵玉米秸秆的过程调控提供基础数据。结果表明,解淀粉芽胞杆菌MN-13可产生纤维素酶,在纤维素—无机盐培养基中发酵60 h,其滤纸纤维素酶(总纤维素酶)的酶活力最高可到8.35 U/mL;在发酵玉米秸秆10 d时半纤维素酶活力达到最高60.46 U/g,纤维素酶活力则在发酵14 d时达到最高45.40 U/g;在发酵19 d时,可溶性糖含量达到最高并趋于稳定;发酵24 d后,菌株对秸秆的组织结构破坏显著,对秸秆纤维素和半纤维素的降解率可分别达到27.12%和40.6%,降解产物中主要包含单糖、醇类和短链脂肪酸等物质。本研究结果为解淀粉芽胞杆菌在生物质资源利用中的应用提供了实验依据。
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杨娟
王艳
蔡云花
李红亚
李术娜
王树香
王全
关键词 解淀粉芽胞杆菌玉米秸秆纤维素半纤维素降解特性    
Abstract:Degradation of corn (Zea mays) stover by microbial fermentation technology is of great significance for realizing the resource utilization of cellulosic biomass. In this study, based on the isolation of a cellulose-degrading strain of Bacillus amyloliquefaciens MN-13 in the early stage of our research group, the cellulase activity of the strain and the degradation characteristics of corn stalk cellulose were further investigated, and while the degradation products of cellulose in stalk were analyzed by gas chromatography-mass spectrometer (GC/MS). The aim of this study is to provide basic data for the process regulation of corn stover fermentation by B. amyloliquefaciens MN-13. The results showed that B. amyloliquefaciens MN-13 could produce cellulases, in which the enzyme activity of filter paper cellulase (total cellulase) reached a maximum of 8.35 U/mL at 60 h in the cellulose-MSM (mineral salt medium) fermentation culture. In the fermentation process of corn stalk cultivated with strain MN-13, the hemicellulase activity reached a maximum of 60.46 U/g at 10th day, and the cellulase activity reached a maximum of 45.40 U/g at 14th day of fermentation. The content of soluble sugar reached the highest level and tended to be stable after fermentation for 19 d. After 24 d of fermentation, the damaged structure of corn stalk was obviously observed, and the degradation rate of cellulose and hemicellulose reaches to 27.12% and 40.6%, respectively. The degradation products mainly contained monosaccharides, alcohols and short-chain fatty acids. The results of this study provide an experimental basis for the study of the process of fermenting corn stalks by Bacillus amyloliquefaciens.
Key wordsBacillus amyloliquefaciens    Corn stalk    Cellulose    Hemicellulose    Degradation characteristics
收稿日期: 2018-09-14     
ZTFLH:  S182  
基金资助:河北省科技支撑计划项目(No. 12226605)
通讯作者: * lihy77@sina.com   
引用本文:   
杨娟, 王艳, 蔡云花, 李红亚, 李术娜, 王树香, 王全. 解淀粉芽胞杆菌对玉米秸秆的降解特性[J]. 农业生物技术学报, 2019, 27(3): 526-533.
YANG Juan, WANG Yan, CAI Yun-Hua, LI Hong-Ya, LI Shu-Na, WANG Shu-Xiang, WANG Quan. Degradation Characteristics of Corn (Zea mays) Straw by Bacillus amyloliquefaciens. 农业生物技术学报, 2019, 27(3): 526-533.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.03.017     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I3/526
 
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