Abstract：Ganjia Tibetan sheep (Ovis aries) grow at high altitude and has a low reproductive rate. The aim of this study was to elucidate the gene expression and tissue distribution of follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in the uterus of the estrous cycle of Ganga Tibetan sheep, to explore the regulation of FSH and LH in Tibetan sheep breeding activities. In the experiment, 32 healthy and non-pregnant Ganjia Tibetan sheep were selected and the uteruses were collected at different stages of the estrous cycle. The detection results of qRT-PCR showed that FSHR and LHR were expressed in uterus tissue during the estrous cycle of Ganjia Tibetan sheep, and the expression level of FSHR was the highest in Estrus, which was significantly higher than that in Proestrus and Metaestrus (P<0.01), and the expression level of LHR was the highest in Diestrus, which was significantly higher than that in Proestrus and Estrus (P<0.01). The detection results of immunohistochemistry showed that FSHR and LHR distributed in the Cervix, Corpus uteri and Cornual uteri of Ganjia Tibetan sheep during estrus cycle, mainly distributed in stromal cells, glandular epithelial cells, vascular endothelial cells and myometrial smooth muscle cells, and FSHR had the most distribution in glandular epithelial cells, stromal cells and myometrial smooth muscle cells in Estrus, and the most distribution in vascular endothelial cells in Diestrus; LHR had the most distribution in glandular epithelial cells, stromal cells and myometrial smooth muscle cells in Diestrus, and the most distribution in vascular endothelial cells in the Metaestrus. Above results indicated that FSHR and LHR might be involved in the regulation of the physiological function of Tibetan uterus during the estrus cycle. This study provides reference data for studying the regulation mechanisms of reproductive activity in Tibetan sheep at the cellular and molecular levels.
王霞, 葛闻博, 何玉琴, 杨大鹏, 周凯仁, 陈卫刚, 包莹莹. 甘加藏羊发情周期子宫中FSHR和LHR表达及分布[J]. 农业生物技术学报, 2019, 27(10): 1885-1893.
WANG Xia, GE Wen-Bo, HE Yu-Qin, YANG Da-Peng, ZHOU Kai-Ren, CHEN Wei-Gang, BAO Ying-Ying. Distribution and Expression of FSHR and LHR in the Uterus of Ganjia Tibetan Sheep (Ovis aries) During Estrus Cycle. 农业生物技术学报, 2019, 27(10): 1885-1893.
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