Abstract：DNA methylation is an essential epigenetic modification closely correlated with the mechanisms underlying cell growth, differentiation and transformation in eukaryotes. Quantifying levels of DNA methylation in tumors is a useful approach for the identification of potential tumor suppressors and to find biomarkers that can be used as prognostic or therapeutic indicators. With the development of technology and further study of DNA methylation, more and more new technologies have been used in the detection of DNA methylation. In this review, this study introduced some typical and novel technologies in different methodologies of DNA methylation analysis. A description of the development of these methods in principle, processes, strengths and weaknesses, optimization and other aspects were given for further study of the detection of of DNA methylation. A description of the development of these methods in principle, processes, strengths and weaknesses, optimization and other aspects were given to offer more options for DNA methylation detection in disease and cancer related study.
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