Abstract：The bait and water quality conditions has important influence on fish producing. In order to explore the effect of different nutrients on several important physiochemical factors of water and the growth of plankton, the variations of dissolved oxygen (DO), ammonia (NH3-N), nitrite (NO2-N), total nitrogen (TN), total phosphorus (TP ) and plankton community were measured after treating the water with inorganic nutrients (IN), micronutrients additives (MA), complex nutritional preparation (CNP) or no fortification (NF). The results of physiochemical factors showed that the IN were extremely improved by DO, NO2-N, NH3-N and TN content (P＜0.01). However, the MA group of NH3-N was only highly significantly improved (P＜0.01), highly significantly decreased NO2-N (P＜0.01), and other physiochemical factors were not significantly different from the NF group. The prokaryotes denaturing gradient gel electrophoresis (DGGE) profiles showed that the NF group of the most bands in detected 44, secondly for the group of IN (37)and MA(36), and the CNP group at least, only 34. Shannon-wiener diversity index(H') and Pielou evenness index(J) were the highest in NF with 3.78 and 0.91, respectively, and the lowest in CNP was 3.53 and 0.85, respectively. The eukaryotes DGGE profiles showed that the CNP group with the largest number by detected 25, the second of NF group (22) and the third of MA group (21), and the CNP group was the least, only 18. H' and J of the CNP group were the largest by 3.22 and 0.86 respectively, while the IN group was the minimum by 2.89 and 0.77, respectively. The test concluded that TN and TP increased significantly in all the treatment group，hazardous substances and harmful bacteria large increased in group treat with IN, while it showed a decrease in group MA; The CNP group had the highest diversity of plankton. Under this experiment condition, the MA group had effective control on the hazardous substances and harmful bacteria of the water, the CNP group could provide the water with abundance nutrients and improve the culture environment. The experimental results will provide theoretical basis to improve the water ecological environment and health cultivation.
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