Correlation Analysis of Single Nucleotide Polymorphisms of Cellular Retionol-binding Protein 4 (CRBP4) and Ovocalyxin-32 (OCX-32) Genes with Egg Production Traits in Lingkun Chicken (Gallus gallus)
Abstract：The cellular retinol-binding proteins (CRBPs) play important roles in intestinal vitamin A absorption and cellular retinol transport due to the extreme hydrophobicity of retinol, and also regulate the metabolism and homeostasis of retinoids through interaction with metabolic enzymes. Ovocalyxin-32 (OCX-32) is a component of the avian uterine milieu present during formation of the eggshell. OCX-32 has attracted much attention for its antibacterial function and potential termination of calcification in egg shell. The purpose of this study was to find the association of single nucleotide polymorphisms of CRBP4 and OCX-32 genes with egg production traits and some important molecular markers for such traits of Lingkun chicken (Gallus gallus). The SNPs in the exon 2, 5 of CRBP4 gene and exon 5 of OXC-32 gene were screened by DNA direct sequencing in 276 individuals of Lingkun chicken. The results showed that there were 3 SNPs (A337C, G2727A and A2847G) in CRBP4 gene, the mutation site of A337C was significantly associated with the total egg number of 300 d (P＜0.05), the mutation sites of G2727A and A2847G were significantly associated with egg shape index and albumen height (P＜0.05). In CRBP4 gene, genotype AA was the advantage genotype. Through inheritance and selection of the AA dominant genotype, there was a potential to further improve Lingkun chicken egg shape index and albumen height. There were 3 SNPs (G6566A, A6612G and A6760T) in the intron 4 of OCX-32 gene, the mutation site of G6566A was significantly associated with the albumen height (P＜0.05), the mutation sites of A6612G and A6760T were significantly associated with yolk weight (P＜0.05). In OCX-32 genotype BB was the advantage genotype. Through inheritance and selection of the BB dominant genotype, there was a potential to further improve Lingkun chicken albumen height and yolk weight. The 6 SNPs were in the Hardy-Weinberg state of equilibrium. The results showed that egg production might be closely related to the expression of CRBP4 and OCX-32 genes, and can be used as a molecular marker for egg production traits in marker assisted selection (MAS) breeding of Lingkun chicken, which provide a theoretical basis for Lingkun chicken breeding.
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