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2025年8月18日 星期一
  2015, Vol. 23 Issue (10): 1343-1349    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
灵昆鸡视黄醇结合蛋白(CRBP4)和蛋壳基质蛋白(OCX-32)基因多态性与产蛋性状的相关性研究
张丽萍1,曾涛2,卢立志3,刘均4,周树和5,林兴钦5,田勇6
1. 浙江省农业科学院畜牧兽医所
2.
3. 浙江省农科院畜牧兽医所
4. 浙江省农科院 畜牧兽医所
5. 温州市金土地农业开发有限公司
6. 浙江省农业科学院畜牧兽医研究所
Correlation Analysis of Single Nucleotide Polymorphisms of Cellular Retionol-binding Protein 4 (CRBP4) and Ovocalyxin-32 (OCX-32) Genes with Egg Production Traits in Lingkun Chicken (Gallus gallus)
1, 1, 1, 1, 1,Yong Tian1
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摘要 细胞视黄醇结合蛋白(cellular retionol-binding protein 4, CRBP4)担任着肠道吸收维生素A和运输细胞视黄醇的重要任务,并通过与代谢酶的相互作用调节类视黄醇的代谢与平衡。蛋壳基质蛋白(ovocalyxin-32, OCX-32)是禽蛋壳形成过程中子宫内的一种组成成分,因其具有抗菌和潜在的终止蛋壳钙化的功能而被广泛关注。为了寻找CPBP4和OCX-32基因多态性与灵昆鸡(Gallus gallus)产蛋性状的相关性,本研究采用DNA直接测序法,对灵昆鸡276个个体的CRBP4基因外显子2、5和OCX-32基因外显子5进行单核苷酸多态性(single nucleotide polymorphisms, SNPs)筛选。结果发现,CRBP4基因存在3个突变位点,即A337C、G2727A和A2847G,其中A337C位点与300日龄灵昆鸡产蛋量显著相关(P<0.05),G2727A和A2847G位点与蛋形指数、蛋白高度显著相关(P<0.05),AA基因型是优势基因型;OCX-32基因内含子4上有3个突变位点,G6566A、A6612G和A6760T,G6566A位点与蛋白高度显著相关(P<0.05),A6612G、A6760T位点与蛋黄重显著相关(P<0.05),BB基因型为优势基因型。6个位点均处于Hardy-Weinberg平衡状态。本研究结果表明,CRBP4和OCX-32基因可能与灵昆鸡的产蛋性状密切相关,可以尝试作为灵昆鸡产蛋性状分子标记辅助育种的候选基因,为灵昆鸡选育工作提供理论依据。
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张丽萍
曾涛
卢立志
刘均
周树和
林兴钦
田勇
关键词 灵昆鸡细胞视黄醇结合蛋白基因(CRBP4)蛋壳基质蛋白基因(OCX-32)单核苷酸多态性(SNPs)产蛋性状    
Abstract:The cellular retinol-binding proteins (CRBPs) play important roles in intestinal vitamin A absorption and cellular retinol transport due to the extreme hydrophobicity of retinol, and also regulate the metabolism and homeostasis of retinoids through interaction with metabolic enzymes. Ovocalyxin-32 (OCX-32) is a component of the avian uterine milieu present during formation of the eggshell. OCX-32 has attracted much attention for its antibacterial function and potential termination of calcification in egg shell. The purpose of this study was to find the association of single nucleotide polymorphisms of CRBP4 and OCX-32 genes with egg production traits and some important molecular markers for such traits of Lingkun chicken (Gallus gallus). The SNPs in the exon 2, 5 of CRBP4 gene and exon 5 of OXC-32 gene were screened by DNA direct sequencing in 276 individuals of Lingkun chicken. The results showed that there were 3 SNPs (A337C, G2727A and A2847G) in CRBP4 gene, the mutation site of A337C was significantly associated with the total egg number of 300 d (P<0.05), the mutation sites of G2727A and A2847G were significantly associated with egg shape index and albumen height (P<0.05). In CRBP4 gene, genotype AA was the advantage genotype. Through inheritance and selection of the AA dominant genotype, there was a potential to further improve Lingkun chicken egg shape index and albumen height. There were 3 SNPs (G6566A, A6612G and A6760T) in the intron 4 of OCX-32 gene, the mutation site of G6566A was significantly associated with the albumen height (P<0.05), the mutation sites of A6612G and A6760T were significantly associated with yolk weight (P<0.05). In OCX-32 genotype BB was the advantage genotype. Through inheritance and selection of the BB dominant genotype, there was a potential to further improve Lingkun chicken albumen height and yolk weight. The 6 SNPs were in the Hardy-Weinberg state of equilibrium. The results showed that egg production might be closely related to the expression of CRBP4 and OCX-32 genes, and can be used as a molecular marker for egg production traits in marker assisted selection (MAS) breeding of Lingkun chicken, which provide a theoretical basis for Lingkun chicken breeding.
Key wordsLingkun chicken    Cellular retionol-binding protein 4 gene(CRBP4)    Ovocalyxin-32 gene (OCX-32)    Single nucleotide polymorphisms (SNPs)    Egg's traits
收稿日期: 2015-04-09      出版日期: 2015-07-24
基金资助:浙江省科学技术厅重大科技专项重大农业项目“肉鸡全产业链质量安全控制与溯源技术体系研究与应用示范”
通讯作者: 田勇     E-mail: 1015080153@qq.com
引用本文:   
张丽萍 曾涛 卢立志 刘均 周树和 林兴钦 田勇. 灵昆鸡视黄醇结合蛋白(CRBP4)和蛋壳基质蛋白(OCX-32)基因多态性与产蛋性状的相关性研究[J]. , 2015, 23(10): 1343-1349.
Yong Tian. Correlation Analysis of Single Nucleotide Polymorphisms of Cellular Retionol-binding Protein 4 (CRBP4) and Ovocalyxin-32 (OCX-32) Genes with Egg Production Traits in Lingkun Chicken (Gallus gallus). , 2015, 23(10): 1343-1349.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2015/V23/I10/1343
 
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