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铅锌矿尾矿坝分离节杆菌12-1对Pb2+的耐受和吸附性能研究
陈志1,邹情雅2,潘晓鸿1,林璋3,关雄4
1. 福建农林大学
2.
3. 中国科学院福建物质结构研究所
4. 福建农林大学生命科学院
Pb2+ Tolerance and Adsorption of Arthrobacter sp. 12-1 Isolated from Lead-zinc Mine Tailing Dam
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摘要 当前铅资源的粗放式开采和使用对环境造成了严重的污染。利用微生物修复铅污染具有费用低、易操作、环境友好等优点,在水体和土壤铅污染治理中具有很好的应用前景。为了了解微生物对铅的吸附特性,本研究从铅锌矿尾矿坝分离到的一株耐铅节杆菌(Arthrobacter sp.)12-1(GenBank登录号: KM362724),并研究其对铅的吸附过程和作用机制。研究耐铅节杆菌 12-1在含不同Pb2+浓度LB培养基中的生长情况表明,其最高可耐受800 mg/L Pb2+。在水溶液中,经24 h吸附,耐铅节杆菌 12-1可将Pb2+浓度从105 mg/L降至2.17 mg/L,吸附率为97.93%。显微成像(原子力显微镜, 扫描电镜)观察和能谱分析表明,耐铅节杆菌 12-1吸附铅后在细胞表面形成含铅的矿物。傅立叶变换红外光谱(FT-IR)分析表明,耐铅节杆菌 12-1细胞上的羧基、酰胺和磷酸基团可能参与了铅的吸附和固定过程。以上结果表明,从铅锌矿尾矿坝分离到的耐铅节杆菌 12-1对铅具有较好的耐受和吸附能力,显示其在铅污染环境修复中具有潜在的应用前景。本研究为细菌修复铅污染环境的实践提供了理论基础。
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陈志
邹情雅
潘晓鸿
林璋
关雄
关键词 节杆菌生物吸附耐受铅锌矿尾矿    
Abstract:The extensive exploitation and usage of lead resources have caused serious environmental pollution problems currently. Bioremediation of Pb2+ contaminated water and soil environments using microbes is regarded as a promising technology due to the advantages of its cost-effective, easy operation and environmental-friendly properties. In order to understand Pb2+ biosorption characterization of microbes, an indigenous lead-resistant bacterium-Arthrobacter sp. 12-1(GenBank No. KM362724) was isolated from lead-zinc mine tailing dam, and the process and mechanism of Pb2+ biosorption by Arthrobacter sp. 12-1 were furtherly investigated in this study. Study on the growth of Arthrobacter sp. 12-1 in LB medium containing different concentration of Pb2+ suggested that the highest Pb2+ tolerant concentration of Arthrobacter sp. 12-1 was 800 mg/L. In water solution, 105 mg/L of Pb2+ could be reduced to 2.17 mg/L by Arthrobacter sp. 12-1 within 24 h with biosorption rate of 97.93%. Microscopic investigation (atomic force microscopy and scanning electronic microscopy) combined with energy dispersive X-ray spectroscopy analysis showed that lead containing mineral was formed on the surface of cell after Pb2+ sorption by Arthrobacter sp. 12-1. Further fourier transform infrared (FT-IR) analysis revealed that carboxyl, amide and phosphate groups of Arthrobacter sp. 12-1 might be involved in Pb2+ biosorption process. The results demonstrated that the Arthrobacter sp. 12-1 isolated from lead-zinc mine tailing dam had strong ability of Pb2+ resistance and biosorption, indicating an attractive prospect of practical applications in bioremediation Pb2+ contaminated water and soil environments. The present work provides much fundamental information for help in constructing feasible strategies for Pb2+ bioremediation in the environment.
Key wordsLead    Arthrobacter sp.    Biosorption    Tolerance    Lead-zinc mine tailing
收稿日期: 2014-07-08     
通讯作者: 关雄   
引用本文:   
陈志1,邹情雅2,潘晓鸿1,林璋3,关雄4. 铅锌矿尾矿坝分离节杆菌12-1对Pb2+的耐受和吸附性能研究[J]. , 2014, 22(11): 1394-1401.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2014/V22/I11/1394
 
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