Expression of Secretory Immunoglobulin A(sIgA) Protein and mRNA in Lung and Trachea of Macaque(Macaca mulatta) Infected Spontaneously Strpetococcus pneumoniae
Abstract：Streptococcus pneumoniae is the importance germ to influence macaques's health. In order to investigate the expression and distribution of secretory immunoglobulin A(sIgA) protein and mRNA in lung and trachea of macaque infected Streptococcus pneumoniae spontaneously, immunohistochemistry and in situ hybridization were used. Results showed that the positive area of sIgA protein in alveolar septum were extremely higher than that of normal group (P<0.01), while in pulmonary vascular wall the difference were significantly higher (P<0.05), the optical density and positive area of sIgA in tracheal epithelium and lamina propria were higher than that of normal group (P<0.05). The expression of sIgA mRNA in alveolar septum, pulmonary intravascular and vessel wall were extremely higher than that of normal group (P<0.01), while in tracheal epithelium was significantly higher (P<0.05); compared with normal group, the optical density in tracheal epithelium and muscular layer were significantly higher than that of normal group (P<0.01), and it was higher in lamina propria of trachea (P<0.05). It can be concluded that sIgA participated the immunomodulation process of Streptococcus pneumoniae infection in lung and trachea. The results give us a new ideas to establish directional treatment and immune way.
