Abstract：In order to understand the internal relationship between the dynamic changes and characterization of microbial iron(Ⅲ) reduction in paddy soil, dynamic changes of community structure of Anaeromyxobacter were analyzed. Paddy soil samples were flooded 1 h, 1 d, 5 d, 10 d, 20 d, and 30 d respectively, and the dynamic changes of Anaeromyxobacter community structure were analyzed with PCR-RFLP technique. The 16S rRNA gene sequences obtained in this study have been submitted to NCBI under accession numbers JF430083~JF4301440. And reduction of Fe(Ⅲ) was determined with anaerobic slurry incubation method. The results showed that α diversity index among different flooding treatments illuminated distinct differences. The diversity was the highest in flooding 20 d treatment and the lowest in 10 d. In all samples, 11 RFLP-based dominant patterns(P1~P11) were found and divided into 3 groups based on 16S rRNA gene phylogenetic analysis. Group 1 and Group 2 included five dominant patterns respectively, one pattern(P1), was shared. And all the patterns in Group 1 and Group 2 were affiliated with Anaeromyxobacter capable of dissimilatory iron reduction. Group 3 was obviously different from the other two. In paddy soil treated with different flooding time, the dynamic succession of Anaeromyxobacter community structure occurred, and Anaeromyxobacter in Group 1 had close relationship with ferrihydrite reduction at flooding early phase and goethite reduction at the late stage. This research provides a theoretical basis to further clarify the environmental function of Anaeromyxobacter in soil.”
