Abstract：The sexual polyploidization through 2n gametes, which could result in intergenomic recombination to produce new genetic variations, is paid more and more attention by plant breeders. In this study, the young flowers of colored Zantedeschia diploid cultivars were treated by the chemical method (colchicines) to induce 2n gamete, and the cytological characteristics of 2n male gamete (2n pollen) formation were observed. The result showed that 2n pollen of coloured Zantedeschia was induced successfully by colchicine and 2n pollen grain was larger than that of normal 1n pollen. The best concentration of colchicines was 0.15%. There are obvious differences on cytological matters during 2n pollen formation. The abnormal phenomena like parallel spindles and fused spindles at metaphase, dyads and triads at the tetrad stage, multinuclear cell with abnormal meiosis, nuclei fusion in pollen mother cell, etc. were observed. The seeds of the treated pollen crossing with normal 1n female gametes were harvested. The chromosome identification showed that the triploid (2n=3X=48) progeny was obtained successfully. The results implied that 2n pollen induced artificially can be a powerful method to create the sexual polyploids of colored Zantedeschia.
