摘要为寻找小菜蛾(Plutella xylostella)重要的生理功能相关基因，以小菜蛾为材料，用SMART(Switching mechanism at 5' end of RNA transcript)技术构建了小菜蛾2龄幼虫cDNA文库，对文库的部分序列进行测序及分析，结果表明，该文库原始库容达到1.1×106，扩增库容达到2.6×109，重组率达到92%，平均插入片段大小为1 kb左右，文库质量达到要求。序列分析得到了小菜蛾的部分新基因，其中部分已登录到GenBank(登录号为 GW883877~GW883893、GU014922)。对其中第11号基因(GenBank登录号：GU014922)表达产物及其二级结构和高级结构域的预测结果显示，该基因可能为丝氨酸蛋白酶家族基因的一个新成员，参与小菜蛾体内重要的代谢、免疫反应。该文库的构建为研究小菜蛾的生理功能相关基因和进一步研究小菜蛾基因功能提供了技术平台。
Abstract：The diamondback moth (Plutella xylostella) is the grisly pests for cruciferous vegetables and crops. In this experiment, a cDNA library of the second instar larvae of diamondback moth was built by SMART (switching mechanism at 5' end of RNA transcript) technology. Sequencing and library analysis showed that the original storage capacity of the full-length cDNA library was 1.1×106, storage capacity of the amplified library was 2.6×109, the recombination frequency was 92%, with an average fragment size around 1 kb. All these indicated that the library quality met the requirements. Sequence analysis showed that the library contained some new genes, and some of them have been submitted in GenBank (Accession No. GW883877~GW883893, GU014922 ). Predicate the protein structure of the gene No. 11(GenBank accession No：GU014922) indicate that the gene belonged to gene family of serine protease, which was concerned with important metabolism and immunoreaction. The construction of the library may benefit for finding new gene and lay a foundation for the further genes function analysis of P. xylostella.
