Abstract:Abstract The wheat accession H9020-20-12-1-8 is a translocation line previously derived from hybridization and backcross between common wheat (Triticum aestivum L.) and Psathyrostachys huashanica Keng. It shows excellent resistance to the dominant races(CYR32) of Puccinia striiform f. sp. tritici prevalent in China at seedlings. The genetic analysis showed that H9020-20-12-1-8 had one dominant gene to CYR32. The resistance gene temporarily was designated as YrHy. A 248 individuals of F2 segregating population derived from H9020-20-12-1-8 and a susceptible wheat line Mingxian 169 was mapped using simple sequence repeat (SSR) markers, three of the 305 SSR markers on 2BS (Xgwm429, Xwgm770 and Xwmc154) were selected and linked with YrHy and with a distance of 5.4,6.4 and 11.3 cM, respectively. YrHy is a novel resistance gene to wheat stripe rust donated from P. huashanica Keng on the basis of pedigree analysis and molecular results.