Abstract：Abstract：Growth differentiation factor9(GDF9)is one of the members of transforming growth factorβ(TGFβ)，which is essential for growth and differentiation of early ovarian follicles，This experiment was conducted to study the Tissue Expression Pattern，mRNA expression levels and SNPS. We use RT-PCR,real time PCR and PCR-RFLP analyse the Tissue Expression Pattern,the mRNA expression levels in the ovary of single fetuses and triplets Hu sheep during oestrum,and the polymorphism at 152 on Exon1 of GDF9 gene.The results indicated that GDF9 gene expresses in Hypothalamus, pituitary, ovary,uterus, Fallopian tube, heart, liver, spleen,lung and kidney of Hu sheep；the mRNA expression levels on GDF9 gene in the ovary of triplets Hu sheep is prominent higher than that in single fetuses Hu sheep(P < 0.05),the single nucleotide mutation:A→G at cDNA 152 of GDF9 gene in Hu sheep is not detected.The analyse results indicated that The mRNA Expression Level of GDF9 gene have some relation with the reproduction power in Hu sheep,and wait for the further analyses of The polymorphism of GDF9 gene in Hu sheep.
