联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月8日 星期五
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
肉鸡H9N2亚型禽流感病毒全基因测序及遗传进化分析
万春和,傅光华,程龙飞,陈红梅,林芳,林建生,黄瑜,施少华
福建省农业科学院畜牧兽医研究所
Phylogenetic analysis of the eight genes of H9N2 subtype avian influenza virus
全文: PDF (698 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 根据GenBank登陆的H9N2亚型禽流感病毒(AIV)的全基因序列,设计11对引物,运用RT-PCR方法扩增A/Chicken/Zhejiang/HJ/2007(H9N2)毒株8个基因片段,并克隆到pMD18-T载体中进行序列测定。基因序列比较及遗传进化分析结果表明,所克隆的8个基因片段均有HJ毒株完整的开放阅读框(ORF),其血凝素(HA)基因蛋白裂解位点的氨基酸组成为-PSKSSR*G-,为不连续的碱性氨基酸,符合低致病性AIV HA基因裂解位点的序列特征;与QA/HK/G1/97的进化关系较近,处在同一分支。其神经氨酸酶(NA)基因在63、64、65位点上有3个氨基酸(T、E、I)缺失,在遗传进化上属于常见的CK/BJ/1/94(DK/HK/Y280/97)群系。核蛋白(NP)基因整合有同地分离的H5N1亚型流感病毒NP基因片段,进化关系较近。基质蛋白(M)基因与1999年香港感染儿童的两株流感病毒(HK/1073/99和HK/1074/99)进化关系属于同一分支。非结构蛋白(NS)基因与近年来分离的猪源H9N2亚型流感病毒进化关系相近,和1998年首次报道的猪源H9N2亚型流感病毒(SW/HK/9/98)处在同一进化分支。聚合酶蛋白(PA、PB2、PB1)中,PB1从33-47位存在15个连续碱基缺失,是首次报道该基因ORF内存在缺失,从遗传进化上和近年来分离的H5N1亚型流感病毒处在同一分支上;PA基因从遗传进化上和PB1基因类似;PB2基因与常见的流感病毒毒株的遗传进化关系较远,在相应的进化树另成分支。因此,A/Chicken/Zhejiang/HJ/2007(H9N2)是由不同流感病毒基因亚系间发生自然重排的产物。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
万春和
傅光华
程龙飞
陈红梅
林芳
林建生
黄瑜
施少华
关键词 H9N2亚型禽流感病毒基因重组进化分析    
Abstract:The eight full-length genes of H9N2 subtype avian influenza virus A/Chicken/Zhejiang/HJ/2007(H9N2) were amplified by reverse transcription-polymerase chain reaction (RT-PCR),and then cloned to pMD18-T vector and sequenced. The sequenced result revealed that the hemagglutinin(HA)gene’s deduced amino acid sequence at the cleavage site of the HA protein was -PSKSSR*G-, which was match to the characteristic of low pathogentic avian influenza(LPAI), which belong to the A/Quail/Hong Kong/G1/97 (H9N2) sublineage, not the same as most of the viruses belong to the A/Chicken/Beijing/1/94 (H9N2) sublineage.The neuraminidase (NA) gene had deletions at 63, 64 and 65 amino acids, which derived from the A/Chicken/Beijing/1/94(H9N2)[A/Duck/HongKong/Y280/97(H9N2)]sublineage.The nucleoprotein (NP) was similar with some H5N1 subtype influenza viruses which also isolated in Zhejiang Province, they all had near phylogenetic relationship. The matrix (M)protein was at one sublineage with A/Hong Kong/1073/99(H9N2) and A/Hong Kong/1074/99(H9N2), those two viruses infected two children in Hong Kong at 1999. The nonstructural (NS) protein was at one sublineage with H9N2 subtype swine influenza viruses isolated during the past few years, at one sublineage with A/Swine/Hong Kong/9/98(H9N2). However, the polymerase protein: the PB1 gene had 15 nucleotides(caatccgactttact)deletions from 33 to 47, which was first report had deletion 5 amino acids from four to eight in ORF. The PA gene and the PB1gene had familiar phylogenetic, which were near to the H5N1 subtype influenza viruses. But the PB2 gene didn’t show any immediate ancestor with the different avian influenza viruses used in the paper. Therefore, A/Chicken/Zhejiang/HJ/2007(H9N2) is product of natural reassortment from different influenza viruses sublineages.
收稿日期: 2008-11-12     
通讯作者: 黄瑜   
引用本文:   
万春和,傅光华,程龙飞,陈红梅,林芳,林建生,黄瑜,施少华. 肉鸡H9N2亚型禽流感病毒全基因测序及遗传进化分析[J]. , 2009, 17(5): 750-757.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2009/V17/I5/750
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn