Construction of cDNA Library and Analysis of Expressed Sequence Tags of Chinese Wild Vitis pseudoreticulata Clone Baihe-35-1 Resistance to Downy Mildew
Abstract：A cDNA library was constructed with Vitis pseudoreticulata clone Baihe-35-1 leaves inoculated by Plasmopara viticola using SMARTTM cDNA Library Construction Kit. The titer of primary library and amplified library was about 0.92×106 pfu/mL and 5×109 pfu/mL, respectively. In the primary library, 97% clones were recombinant and the length of insert cDNAs were from 500 to 2 200 bp. Two hundred and fifty-four ESTs (GenBank accession No. FG269201~FG269449 and FG396006~FG396010) were obtained by random sequencing of the cDNA library. BlastX search showed that 56.3% ESTs had higher homology with functional genes, 14.6% ESTs with hypothetical putative or unknown protein, and 20.9% ESTs had no-hits.
