联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月5日 星期六
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
5种笛鲷属鱼类的遗传多样性及分子标记
刘丽;刘楚吾
广东海洋大学水产学院,湛江 524025
Genetic Diversity and Molecular Markers of 5 Species of Snappers
LIU Li;LIU Chu-wu
Fisheries College , Guangdong Ocean University, Zhanjiang  524025, China
全文: PDF (650 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 运用RAPD及SSR技术对笛鲷属的画眉笛鲷(Lutjanus vitta)、金带笛鲷 (L. fulvus)、金焰笛鲷 (L. fulviflamma)、千年笛鲷(L. sebae)和星点笛鲷(L. stellatus)等5种鱼的遗传多样性及其分子标记进行了研究。RAPD研究结果表明, 5种鱼的平均多态性位点比率(P)依次为89.30%、86.70%、92.11%、86.47%和86.00%; 种内两个体间平均遗传距离(D)分别为0.3431,0.2130,0.3121, 0.1825和0.1775;平均遗传多样性指数(Hi)分别为0.1634、0.1095、0.1353、 0.1022和0.1024。引物OPA8和OPP10的扩增产物中得到9个RAPD标记,分别为OPA8-413bp、OPA8-140bp、OPP10-418bp、OPA8-697bp、OPP10-526bp、OPA8-361bp、OPP10-449bp、OPA8-311bp和OPP10-599b,可鉴别5种鱼。SSR研究结果显示, 5种鱼的有效等位基因数依次为1.9610、3.3793、3.2957、1.7893和3.6591;群体的杂合度(H)分别为0.332、0.462、0.593、0.367和0.676;多态信息含量(PIC)分别为0.302、0.438、0.554、0.332和0.641。 在11个微卫星位点上得到6个微卫星标记,分别为13 Prs229-115 bp、4 Lca43-212 bp、4 Lca43-240 bp、13 Prs229-288 bp、19 Prs275-156bp和7Lca91-118 bp可用于鉴别5种鱼。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
刘丽
刘楚吾
关键词 遗传多样性 分子标记 RAPD SSR 画眉笛鲷 金带笛鲷 金焰笛鲷    
AbstractIn order to protect and develop the valuable fishes of snappers, genetic diversity and molecular markers of  5 species of snappers, Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus,were detected using RAPD and SSR technique. The amplified products of genomic DNA in the 5 species of snappers by RAPD technique indicated that the polymorphic loci ratio (P ) were 89.30%,86.70%,92.11%,86.47% and 86.00%, the mean genetic distances of intra-species (D) were 0.3431,0.2130,0.3121, 0.1825 and 0.1775, and the genetic diversity indexes of intra-species (Hi ) were 0.1634,0.1095,0.1353, 0.1022 and 0.1024 respectively. Nine molecular markers, OPA8-413bp, OPA8-140bp, OPP10-418bp, OPA8-697bp, OPP10-526bp, OPA8-361bp, OPP10-449bp, OPA8-311bp and OPP10-599b , which could  be used for identifying 5 species of snappers were found in the amplified products by primer of OPA8 and OPP10. The amplified products of genomic DNA in 5 species of snappers by SSR technique showed that effective numbers of allelic was 1.9610,3.3793,3.2957,1.7893 and 3.6591 respectively, average heterozygosities were 0.332,0.462,0.593,0.367 and 0.676, polymorphism information contents (PIC) were 0.302,0.438,0.554,0.332 and 0.641 respectively. Six molecular markers, 13 Prs229-115 bp, 4 Lca43-212 bp, 4 Lca43-240 bp, 13 Prs229-288 bp, 19 Prs275-156bp and 7Lca91-118 bp, which could be used for identifying 5 species of snappers were found in 11 loci of micrrosatellite.
Key wordsgenetic diversity    molecular marker    RAPD    SSR    Lutjanus vitta    L. fulvus    L. fulviflamma
收稿日期: 2005-05-20     
ZTFLH:  S184  
  S188  
通讯作者: 刘楚吾   
引用本文:   
刘丽;刘楚吾 . 5种笛鲷属鱼类的遗传多样性及分子标记[J]. , 2006, 14(3): 349-355.
LIU Li;LIU Chu-wu. Genetic Diversity and Molecular Markers of 5 Species of Snappers . , 2006, 14(3): 349-355.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2006/V14/I3/349
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn