Effect of 60Co-γ Rays on In vitro Mutagenesis of Chrysanthemum morifolium Ramat
WANG Jing;LIU Lu-xiang;ZHAO Shi-rong;GUO Hui-jun;ZHAO Lin-shu;CHEN Wen-hua
Key Laboratory of Agricultural Nuclear Technology & Space Breeding, Ministry of Agriculture; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：The microshoots of in vitro-cultured Chrysanthemum morifolium cv. Qiuzhishan were irradiated with 5~30 Gy 60Co-γ rays. After irradiation the microcuttings were transferred to root-inducing medium and subculture medium respectively to study the inductive effects of γ rays on rooting, shoot growth and multiplication on media. The plant growth and flower characters were investigated after the regenerated plantlets were transplanted out of the protected aseptic environment. The results showed that 20 Gy was the lethal dose of 60Co-γ rays to irradiate the microshoots of in vitro -cultured Chrysanthemum morifolium cv. Qiuzhishan, while 10 Gy was the optimum dose to induce mutations. After bloom, 8.2% of the plants in the treatment of 10 Gy were selected for their flower color mutations. In contrast with the bright yellow flowers of the control, different shades of red color were observed in those mutant flowers. All the mutant plants were homogeneous and stable mutants.
