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2025年8月11日 星期一
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
半夏的组织培养及其成分比较
郭余龙 贾永芳 杨星勇* 李名扬 裴 炎
(西南农业大学生物技术研究中心, 重庆 400716)
Tissue Culture and Component Analysis of Pinellia ternata
Guo Yulong Jia Yongfang Yang Xingyong* Li Mingyang Pei Yan
(Research Center of Biotechnology, Southwest Agriculture University, Chongqing 400716, China)
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摘要 摘要:三叶半夏(Pinellia ternata Breit. )叶柄切段在含BA 0.5~2.0 mg/L和NAA 0.2 mg/L的MS培养基上,诱导小块茎的频率最高可达100%,小块茎用BA 2.0 mg/L+NAA 0.2 mg/L的MS培养基增殖,3周内增殖频率达16.9倍,小块茎易于生根移栽。应用组织培养技术大批量生产优质半夏种苗是可行的。组织培养和野生的块茎分别用80%甲醇、0.2 mol/L硫酸、去离子水提取内含物,经HPLC分析,比较其图谱表明, 组织培养块茎中各成分的总含量高于野生块茎,二者的主成分构成相似。
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郭余龙贾永芳杨星勇*李名扬裴炎
关键词 关键词:三叶半夏 组织培养 化学成分 工业化生产    
AbstractAbstract: Pinellia ternata Breit, a Chinese traditional herb, is facing problems of resource declining and requirement increasing.  The segments of leaves and petioles from P. ternata were used as explants to induce small tuber. The highest inducing frequency reached 100% on MS medium supplemented with BA 0.5~2.0 mg/L and NAA 0.2 mg/L. One tuber propagated to 16.9 tubers evenly within 3 weeks on MS medium with BA 2.0 mg/L and NAA 0.2 mg/L.The small tubers could be easily induced to plantlets and transplanted to field. The experiment showed that it was practicable to produce large numbers of high quality plantlets by tissue culture. HPLC was employed to analyze the extracts of 80% methanol, 0.2 mol/L H2SO4 and distilled water from wild and tissue culture tubers. The results showed that the total content of the components tested in the tissue culture tubers were higher than that in the wild, and the main chemical composition were similarity between them.
Key wordsKey words: Pinellia ternata    tissue culture    chemical composition    industrial produce
收稿日期: 1900-01-01     
通讯作者: 杨星勇*    
引用本文:   
郭余龙 贾永芳 杨星勇* 李名扬 裴 炎. 半夏的组织培养及其成分比较[J]. , 2003, 11(3): 259-262.
Guo Yulong Jia Yongfang Yang Xingyong* Li Mingyang Pei Yan . Tissue Culture and Component Analysis of Pinellia ternata . , 2003, 11(3): 259-262.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2003/V11/I3/259
 
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