Regeneration of Interspecific Somatic Hybrid between Sweetpotato and Its Wild Relative Ipomoea triloba*
Wang Jingshan1 Liu Qingchang2** Meng Xiangxia1 Wang Weihua1 Xu Lijuan1 Zhai Hong2
(1. Department of Agronomy, Laiyang Agricultural College, Shandong Laiyang 265200, China; 2. Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China)
Abstract：Abstract: Protoplasts of sweetpotato (Ipomoea batatas ) cv. Genki were fused with protoplasts of its wild relative I. triloba by the PEG method. The fusion products were cultured in a modified liquid MS medium containing 0.05～0.20 mg/L 2,4-D and 0.5 mg/L KT. After 7 weeks of culture, small calluses 1～2 mm in size were transferred to solid MS medium supplemented with 0.05～0.20 mg/L 2,4-D and 0～0.5 mg/L KT for callus proliferation. When the calluses were transferred either to the basal medium or to the medium supplemented with 3.0 mg/L BAP and further to the basal medium, 69 plants were regenerated. PCR analysis indicated that 1 of 69 regenerated plants was the somatic hybrid. This somatic hybrid showed the climbing habit looked like I. triloba and the stem color, green to purple, looked like Genki. The internode length and the leaf shape were intermediate. The chromosome number of root tip cells ranged from 54 to 103, depending on different root tip cells.
