Effect of YWHAZ Gene on the Proliferation and Pigration of Prostate Cancer 22RV1 Cells
LI Yong1, ZHAO Jia-Fu2, LUO Bin-Jie1, CHEN Chen2, SONG Lin-Jin2, XU Hou-Qiang1,2,*
1 Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEA), Institute of Agro-bioengineering/College of Life Sciences, Guizhou University, Guiyang 550025, China; 2 Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction, Ministry of Education, Guizhou University, Guiyang 550025, China
Abstract：Prostate cancer is a heterogeneous disease with a high degree of clinical and genetic heterogeneity, thus providing multiple therapeutic targets.To investigate the expression profiles of 14-3-3 protein family isoforms in different cell lines of prostate cancer and to search for specific drug targets for the treatment of prostate cancer.The expression patterns of each isoform of the 14-3-3 protein family in different prostate cancer cell lines were analyzed by qPCR (real-time fluorescence quantitative PCR) in combination with the UALCAN-TCGA database. The effects of different expression levels on the proliferation and migration ability of 22RV1 cells were demonstrated by up- and down-regulating the expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) gene using CCK-8 and cell scratch assay. It was found that the expression of YWHAZ in cancer tissues was lower than that in normal tissues when GLEASON SCORE was lower than 8, while the relative expression of YWHAZ in prostate cancer cell lines showed a significant upward trend in the middle and late stages as Gleason score (GS) was elevated. The results of CCK-8 assay and cell scratch assay revealed that the interference and overexpression of 14-3-3ζ expression levels in 22RV1 cells severely affected the proliferation and migration ability of the cells YWHAZ. The above results implied that YWHAZ may play a role as a proto-oncogene in the middle and late stages of prostate cancer development. The related results provide a theoretical basis and experimental ideas for the subsequent study of drugs targeting this gene.
李永, 赵佳福, 罗斌杰, 陈晨, 宋林锦, 许厚强. YWHAZ基因对前列腺癌22RV1细胞增殖、迁移的影响[J]. 农业生物技术学报, 2022, 30(3): 473-484.
LI Yong, ZHAO Jia-Fu, LUO Bin-Jie, CHEN Chen, SONG Lin-Jin, XU Hou-Qiang. Effect of YWHAZ Gene on the Proliferation and Pigration of Prostate Cancer 22RV1 Cells. 农业生物技术学报, 2022, 30(3): 473-484.
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