Effect of Endogenous H2S on Selenium Tolerance in Brassica rapa ssp. chinensis and Cloning and Analysis of Related Genes
XIN Ai-Jing1,3, YANG Hui-Min1, XUE Yan-Feng2, LIU Xiao-Li1, XIA Qian-Wei2, WANG Yong-Zhu1, SHI Zhi-Qi2, CHEN Jian2, YANG Li-Fei1,3,*
1 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Food Quality and Detection, Jiangsu Academy of Agriculture Science, Nanjing 210014, China; 3 Hexian New Rural Development Research Institute, Nanjing Agricultural University, Hexian 238200, China
Abstract：The industrial pollution and the overuse of selenium fertilizer result in selenium pollution in some area, which further inhibit plant growth. Hydrogen sulfide (H2S), a kind of gaseous signaling molecule in plants, regulates plant development and stress responses. In order to explore the function of H2S synthesis related genes LCDs/DCDs, In this study, Brassica campestris ssp.chinensis was used to study response of the endogenous H2S and its related genes to selenium stress. The root length and plant height of seedlings at different selenium concentrations were measured, it was found that selenium stress significantly inhibited the growth of seedlings, and showed concentration and time effects. The effect of H2S on oxidative damage induced by selenium stress was analyzed by lipid peroxidation and loss of membrane integrity and WSP-1 fluorescent probe technology, the results showed that enhancing endogenous H2S alleviated selenium-induced oxidative injury in seedlings. qRT-PCR analysis showed that selenium stress induced up-regulation of 12 H2S-producing family genes (BrLCD1~BrLCD10, BrDCD1~BrDCD2) in the early stage, which might result in the rapid production of endogenous H2S in the early period of selenium stress. Finally, the full-length cDNA of H2S-producing genes with relative high expression level in response selenium stress were cloned. Sequence analysis showed that the amino acid sequences encoded by these genes contained the typical characteristics of conserved cysteine dehydrogenase. The results of this study provides new evidences for the revealing the mechanisms of plant response to selenium stress.
辛爱景, 杨会敏, 薛延丰, 刘晓丽, 夏芊蔚, 王永竹, 石志琦, 陈健, 杨立飞. 内源H2S对不结球白菜硒耐受的影响及相关基因克隆与分析[J]. 农业生物技术学报, 2022, 30(2): 260-271.
XIN Ai-Jing, YANG Hui-Min, XUE Yan-Feng, LIU Xiao-Li, XIA Qian-Wei, WANG Yong-Zhu, SHI Zhi-Qi, CHEN Jian, YANG Li-Fei. Effect of Endogenous H2S on Selenium Tolerance in Brassica rapa ssp. chinensis and Cloning and Analysis of Related Genes. 农业生物技术学报, 2022, 30(2): 260-271.
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