Study on Phosphorus Solubilization Characteristics of Talaromyces sp. WR1-4 in Soybean (Glycine max) Rhizosphere
REN Qing-Xin1, WANG Ji-Hua1,*, ZENG Qi1, ZHANG Bi-Xian2, LIU Xiu-Lin2
1 School of Life Science and Technology, Harbin Normal University, Harbin 150025, China; 2 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
Abstract：Phosphorus solibilizating fungus can convert the potentially ineffective phosphorus into available phosphorus in the soil, which is an effective way to alleviate the phenomenon of soil phosphorus deficiency and increase the utilization rate of phosphorus fertilizer. The strain WR1-4 (Talaromyces sp.) was screened from the rhizosphere soil of soybean (Glycine max) in preliminary work. In order to clarify its phosphorus solubility and acid production, the phosphate solubilizing ability of strain WR1-4 was measured by plating method and broth method in present study; the molybdenum antimony anti colorimetric method was used to detect the phosphorus solubilizing characteristics of strain WR1-4 under different conditions of insoluble phosphorus, carbon and nitrogen sources; HPLC was used to detect the organic acids produced by WR1-4. The results showed that WR1-4 had different ability to dissolve 5 insoluble phosphates (calcium phosphate, zinc phosphate, aluminum phosphate, phosphate rock powder, iron phosphate), and the effective phosphorus increments were 693.07, 613.47, 260.14, 137.84 and 386.33 mg/L, respectively. Strain WR1-4 used 6 carbon sources in the order of glucose, sucrose, maltose, starch, lactose and cellulose. As to different nitrogen sources, strain WR1-4 had strongest phosphate solubilizing capacity under the condition of ammonium sulfate, and least phosphate solubilizing ability under the condition of potassium nitrate. Strain WR1-4 could produce 8 kinds of organic acids, including oxalic acid, lactic acid, citric acid, malic acid, acetic acid, fumaric acid, tartaric acid, and maleic acid. According to Pearson analysis, the correlation between the increase of available phosphorus and organic acid was positively correlated. At the same time, while strain WR1-4 was in the stage of phosphate solubilization, the pH of the environment decreased. Above results indicate that strain WR1-4 had a strong ability to dissolve phosphorus and a wide range of growth and metabolism conditions, which makes it has potential application prospects in microbial fertilizer development and agricultural production.
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