Investigation on Infection and Genetic Evolution Analysis of Avian Leukemia Subgroup J in the Local Breed Chicken of Zhejiang Province
NI Zheng1, CHEN Liu1, TANG Ao-Xing2, TANG Jing-Yu2, YUN Tao1, LIU Guang-Qing2,*, ZHANG Cun1,*
1 Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China; 2 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Abstract：Subgroup J of avian leukemia is a new subgroup of avian leukemia caused by Avian leukemia virus subgroup J (ALV-J) isolate from broilers (Gallus gallus). Clinically, it mainly causes the growth and immune suppression of diseased poultry, the prevalence of the disease has brought serious harm to the poultry industry. In this study, ELISA method was used to detect the infection of ALV-J in the local breed chicken in GuangDa Poultry Farm in Hangzhou, Zhejiang Province, and the virus was isolated and identified from positive samples. By amplifying the GP85 gene of the isolate, the genetic evolution of the isolated ALV was analyzed. The results showed that the positive rate of ALV-J among 10 local breeds was very different, with the lowest rate being only 4%, and the highest rate being 46%. 3 strains of ALV-J (named ZJ20-1, ZJ20-2 and ZJ20-3, respectively) were isolated from the positive samples using primary chicken embryo fibroblasts (CEFs). The GP85 specific fragment was amplified from the 3 isolates by PCR method, and the genetic evolution of GP85 gene was analyzed by DNA STAR software. The results showed that the ALV-J isolates isolated in this study had a certain genetic evolution relationship with the reference strains. The ALV-J isolates could be divided into 2 topological groups (G1 and G2), and the 3 isolated strains belonged to G1 group. The homology with other domestic isolates was 88%~98.0%. This study provides a reference for further study on the prevalence of ALV-J in chicken farms in China and the purification of avian leukemia.
[1] 崔治中, 张志, 杜岩, 等. 2002. 我国肉用型鸡中J亚群禽白血病的流行现状调查[J]. 中国预防兽医学报, 24(4): 292-294. (Cui Z Z, Zhang Z, Du Y, et al .2002. Epidemiological studies of subgroup J avian XXXeucosis in China[J]. Chinese Journal of Preventive Veterinary Medicine, 24(4): 292-294.) [2] 杜岩, 崔治中, 秦爱建, 等. 2000. 鸡的J亚群白血病病毒的分离及部分序列比较[J]. 病毒学报, 16(4): 341-346 . (Du Y, Cui Z Z, Qi A J, et al.2000. Isolation of subgroup J Avian leukosis viruses and their partial sequence comparison[J]. Chinese Journal of Virology, 16(4): 341-346.) [3] 沈海玉, 窦新红, 许明. 2018. 地方特色蛋鸡配套系亲本群中不同亚群禽白血病病毒的混合感染[J]. 中国家禽, 40(16): 25-28. (Shen H Y, Dou X H, Xu M.2018. Mixed infection of different subgroups of Avian leucosis virus in parent groups of local laying hens[J]. China Poultry, 40(16): 25-28.) [4] 王增福, 崔治中. 2015. 我国1999-2003年间ALV-J野毒株gp85基因变异趋势[J]. 中国病毒学报, 8, 20(4): 393-398. (Wang F Z, Cui Z Z.2015. The mutation tendency of the gp85 gene of chinese field strains of ALV-J from1999 to 2003[J]. Virologica Sinica, 8, 20(4): 393-398.) [5] 徐镔蕊, 董卫星, 余春明, 等. 2005. 用ALV-J gp85单克隆抗体证明蛋鸡存在J亚群禽白血病[J]. 畜牧兽医学报, 36(3): 269-271. (Xu B R, Dong W X, Yu C M, et al .2005. Occurrence of ALV-J in egg-type chickens certif ied bymonoclonal antibody against ALV-J gp85[J].Acta Veterinaria et Zootechnica Sinica, 36(3): 269-271.) [6] 殷震, 刘景华. 1997. 动物病毒学[M]. 科学出版社, 北京, pp. 870-885. (Ying Z, Liu J H.1997. Animal Viology[M]. Science Press.. Beijing, pp. 870-885.) [7] 张会永, 吴井生, 李国辉, 等. 2018. 禽白血病病毒在惠阳胡须鸡和汶上芦花鸡的垂直传播特性分析[J]. 畜牧兽医学报, 49(7): 1475-1481. (Zhang H Y, Wu J S, Li G H, et al.2018. Characteristic analysison vertical transmission of Avian leukosis virus in Huiyang chicken and Wenshang barred chicken[J]. Chinese Journal of Animal and Veterinary Sciences, 49(7): 1475-1481.) [8] 赵冬敏, 张青婵, 崔治中. 2010. 芦花鸡中B亚群禽白血病病毒的分离与鉴定[J]. 病毒学报, 26(1): 53-57. (Zhao D M, Zhang Q C, Cui Z Z.2010. Isolation and identification of a subgroup B Avian leukosis virus from chickens of chinese native breed luhua[J]. Chinese Journal of Virology, 26(1): 53-57.) [9] 郑麦青,李鹏,刘泽,等. 2018. 广东地区规模化鸡场地方鸡种禽白血病净化的初步研究[J].中国兽医学报,37(12): 2301-2305. (Zheng M Q, Li P, Li Z, et al.2018. Eradication effect of avian leukosis for large scale chicken farm in Guangdong province[J]. Chinese Journal of Veterinary Science, 37(12): 2301-2305. [10] Arshad S S, Howes K, Barron G S, et al.1997. Tissue tropism of the HPRS2103 strain of J subgroup Avian leukosis virus and of a derivative acutely transforming virus[J]. Veterinary Pathology, 34(2): 127-137. [11] Bates P, Rong L J, Varmus H E, et al.1998. Genetic mapping of the cloned subgroup a avian sarcoma and leukosis virus receptor gene to the TVA locus[J]. Journal of Virology, 72(3): 2503-2508. [12] Bova H C, Olsen J C, Swanstrom R.1991. Genetic analysis of the Rous sarcoma virus subgroup D env gene: Mammol/ Lal tropism correlates with temperature sensitivity of gp85[J]. Journal of Virology, 65(4): 2073-2080. [13] Davidson I, Borenshtain R.2002. The feather tips of commercial chickens are a favorable source of DNA for the amplification of Marek's disease virus and Avian leucosis virus subgroup[J]. Avian Pathology, 31(3): 237-240. [14] Dorner A J, Coffin J M.1986. Determinants for receptor interaction and cell killing on the Avian retrovirus glycoprotein gp85[J]. Cell, 45(3): 365-374. [15] Gao Y, Qin L, Pan W, et al.2010. Avian Leukosis virus subgroup J in layer chickens, China[J]. Emerging Infectious Diseases, 16(10): 1637-1638. [16] Han C Y, Hao R J, Liu L L, et al.2015. Molecular characterization of 3'UTRs of J subgroup Avian leukosis virus in passerine birds in China[J]. Archives of Virology, 160(3): 845-849. [17] Liu H M, Ma K, Liu M M, et al.2019. Histologic findings and viral antigen distribution in natural coinfection of layer hens with subgroup J Avian leukosis virus, Marek' s disease virus, and Reticuloendotheliosis virus[J]. Journal of Veterinary Diagnostic Investigation, 31(5): 761-765. [18] Payne L N, Brown S R, Bumstead N, et al.1991. A novel subgroup of exogenous Avian leukosis virus in chickens[J]. Journal of General Virology, 2(4): 801-807 [19] Payne L N, Gillespie A M, Howes K.1992.Myeloid leukaemogenicity and transmission of the HPRS-103 strain of Avian leukosis virus[J]. Leukemia, 6(11): 1167-1176. [20] Ruddell A.1995. Transcription regulatory elements of the avian retro-viral long terminal repeat[J]. Virology, 206(1): 1-7. [21] Su Q, Li Y, Cui Z Z, et al.2018a. The emerging novel Avian leukosis virus with mutations in the pol gene shows competitive replication advantages both in vivo and in vitro[J]. Emerging Microbes & Infections, 7(1): 117 [22] Su Q, Li Y, Li W H, et al.2018b. Molecular characteristics of Avian leukosis viruses isolated from indigenouschicken breeds in China[J]. Poultry Science, 97(8): 2917-2925 [23] Sun S H, Cui Z Z.2007. Epidemiological and pathological studies of subgroup J Avian leukosis virus infections in Chinese local “yellow” chickens[J]. Avian Pathology Journal of the Wvpa, 36(3): 221-226. [24] Xu B R, Dong W X, Yu C M, et al.2004. Occurrence of Avian leukosis virus subgroup J in commercial layer flocks in China[J]. Avian Pathology, 33(1):13-17. [25] Zhang Y, Yu M M, Xing L X, et al.2020. The bipartite sequence motif in the N and C termini of gp85 of subgroup J Avian leukosis virus plays a crucial role in receptor binding and viral entry[J]. Journal of Virology, 94(22): e01232-20.
