Effector Screening of Puccinia triticina by Nicotiana benthamiana
QI Yue1, ZHANG Yue1, LI Jian-Yuan1,2, YANG Wen-Xiang1,*, LIU Da-Qun3,*
1 Department of Plant Pathology, Hebei Agricultural University/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China; 2 Hebei Xingtai College, Xingtai 054000, China; 3 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：Wheat (Triticum aestivum) leaf rust is an airborne fungal disease caused by Puccinia triticina, which occurs worldwide and causes yield loss up to 15%~40% or even more in wheat production. Utilization of resistance cultivars is the most effective, safe and economic method. However, the pathogenicity of Puccinia triticina is diversified and the toxicity is frequently mutated. Therefore, the wheat leaf rust resistance is continuously lost. To explore the molecular mechanism of wheat leaf rust pathogenesis is particularly important for the effective control of wheat leaf rust. In this study, based on a total of 9 samples transcriptome data of P. triticinia monospora 08-5-9-2 (KHTT), 13-5-28-1 (JHKT) and 13-5-72-1 (THSN) in dormant spore, germinating summer spore and haustorium formation period, effector proteins were initially screened by SignalP 4.1、TargetP 1.1, TMHMM 2.0 and EffectorP 2.0. Effector proteins of 357 candidates were obtained, and 20 candidate effector proteins were screened for their gradually up-regulated expression level during the interaction between Puccinia triticina and susceptible material Thatcher. The 20 candidate effector proteins were further screened by Nicotiana benthamiana and Bax and INF1 which could cause allergic necrosis, and results showed that the 20 candidates were all effector proteins. The above results showed that the heterologous expression system was a powerful tool for screening the effector protein of specific parasite, which could provide reference for further research on the pathogenicity of effector proteins.
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